Activated microglial supernatant induced motor neuron cytotoxicity is associated with upregulation of the TNFR1 receptor

Neuroscience Research
Weiyan WenMichael J Strong

Abstract

We have previously reported that supernatant derived from LPS-activated BV-2 cells, an immortalized microglial cell line, induces death of NSC-34 cells (a motor neuron hybridoma) through a TNFalpha and nitric oxide synthase (NOS) dependant mechanism. In this study, we have observed that LPS-activated BV-2 supernatant induces NSC-34 cell death in association with an upregulation of the TNF receptor 1 (TNFR1) expression on NSC-34 cells, both at the transcription level and at the cell surface protein level. The upregulation of TNFR1 receptor was independent of TNFalpha, and could be partly inhibited by the inhibition of iNOS activation in the BV-2 cells. The TNFR2 receptor was not involved. These observations have important implications in understanding the mechanism by which microglial activation contributes to the motor neuron degeneration.

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Citations

Mar 17, 2009·Journal of Neuroimmunology·Saima HumayunMichael J Strong
Jul 16, 2008·Physical Medicine and Rehabilitation Clinics of North America·Elsa RaibonThomas Möller
Aug 20, 2008·Fundamental & Clinical Pharmacology·Garima DuttaBin Liu
Jan 24, 2009·Annals of the New York Academy of Sciences·Zala Jenko PraznikarJoze Pungercar
May 15, 2010·Glia·Brandi J BakerEtty N Benveniste
Jun 30, 2021·Neurochemistry International·Ivan A LindhoutAndis Klegeris
Dec 2, 2014·Journal of Proteome Research·Christoph Garbers, Stefan Rose-John

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