PMID: 7545186Jun 1, 1995Paper

Activated phenotype in neutrophils and monocytes from patients with primary proliferative polycythaemia

Journal of Clinical Pathology
N B WestwoodT C Pearson

Abstract

To investigate whether monocytes and neutrophils from patients with primary proliferative polycythaemia (PPP) exhibit increased expression of markers of cell activation and, if so, whether they are associated with the phagocytic activity of these cells and concentrations of circulating cytokines. Expression of CD11b, CD14, CD18, and CD64 on monocytes and neutrophils was assessed by flow cytometry. Phagocytosis was analysed using immunoglobulin opsonised Escherichia coli. Serum concentrations of granulocyte colony stimulating factor (G-CSF), granulocyte-macrophage CSF (GM-CSF) and macrophage CSF (M-CSF) were determined by bioassays, and interferon-gamma (IFN-gamma) by enzyme linked immunosorbent assay (ELISA). Patients with PPP (n = 18), when compared with normal subjects (n = 10), had increased percentages of CD64+ monocytes (52% v 36%) and neutrophils (42% v 11%) and of CD14+ neutrophils (36% v 18%). Monocytes from patients with PPP exhibited increased expression of CD64 (47 v 26) and of CD11b (65 v 36). These abnormalities were not found in patients with secondary (n = 8) or apparent (n = 13) polycythaemia. The percentage of neutrophils undergoing phagocytosis was higher in patients with PPP (mean 64%; n = 6) than in normal s...Continue Reading

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Citations

Sep 12, 2003·Trends in Immunology·Amit R Ashtekar, Bhaskar Saha
Mar 9, 2012·Biochemistry Research International·Simon A YoungTerry K Smith
Aug 1, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Johannes J M L Hoffmann
Nov 3, 2009·Haematologica·Francisco CervantesAlberto Alvarez-Larrán
Jan 14, 2004·British Journal of Haematology·Alberto Alvarez-LarránFrancisco Cervantes
Mar 3, 1998·Blood Reviews·S HinshelwoodA R Green
Aug 15, 1998·Annals of the New York Academy of Sciences·J Samuelsson, J Palmblad
Apr 19, 2005·Expert Review of Molecular Diagnostics·Bruce H Davis
Jun 8, 2011·International Journal of Laboratory Hematology·H FujitaS Nishimura

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