Activated polymorphonuclear cells promote injury and excitability of dorsal root ganglia neurons.

Experimental Neurology
S K ShawCarl Y Saab

Abstract

Therapies aimed at depleting or blocking the migration of polymorphonuclear leukocytes (PMN or neutrophils) are partially successful in the treatment of neuroinflammatory conditions and in attenuating pain following peripheral nerve injury or subcutaneous inflammation. However, the functional effects of PMN on peripheral sensory neurons such as dorsal root ganglia (DRG) neurons are largely unknown. We hypothesized that PMN are detrimental to neuronal viability in culture and increase neuronal activity and excitability. We demonstrate that isolated peripheral PMN are initially in a relatively resting state but undergo internal oxidative burst and activation by an unknown mechanism within 10 min of co-culture with dissociated DRG cells. Co-culture for 24 h decreases neuronal count at a threshold<0.4:1 PMN:DRG cell ratio and increases the number of injured and apoptotic neurons. Within 3 min of PMN addition, fluorometric calcium imaging reveals intracellular calcium transients in small size (<25 microm diam) and large size (>25 microm diam) neurons, as well as in capsaicin-sensitive neurons. Furthermore, small size isolectin B4-labeled neurons undergo hyperexcitability manifested as decreased current threshold and increased firing...Continue Reading

References

Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·V L DawsonS H Snyder
May 1, 1986·The Anatomical Record·H Schmalbruch
Aug 1, 1997·Neuroscience·Y TaokaK Takatsuki
Jan 23, 1999·Journal of Neuroscience Methods·B SchutteF Ramaekers
May 7, 1999·Stroke; a Journal of Cerebral Circulation·C J PrestigiacomoD J Pinsky
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·G J Bennett
Aug 11, 1999·Trends in Neurosciences·U DirnaglM A Moskowitz
Jun 8, 2000·The Journal of Comparative Neurology·T TandrupR E Coggeshall
Dec 13, 2000·Neuroscience·N M Perkins, D J Tracey
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Jun 22, 2002·Annals of the New York Academy of Sciences·Bin LiuJau-Shyong Hong
Sep 25, 2002·Physiological Reviews·Linda R Watkins, Steven F Maier
May 3, 2003·Nature Reviews. Neuroscience·Eng H LoMichael A Moskowitz
Aug 5, 2003·Experimental Brain Research·Carl Y SaabStephen G Waxman
Nov 19, 2003·Archives of Neurology·Robert H DworkinSharon M Weinstein
Dec 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Klaus DinkelRobert M Sapolsky
Jul 9, 2004·Journal of Leukocyte Biology·Mark T Quinn, Katherine A Gauss
Oct 27, 2004·Anesthesiology·Michael E JohnsonGregory J Gores
Mar 11, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ron AmirMarshall Devor
Mar 18, 2005·Annual Review of Immunology·Anthony W Segal
Jul 5, 2005·Nature Reviews. Neuroscience·Fabien MarchandStephen B McMahon
Jul 19, 2005·The Journal of Biological Chemistry·Tianxin YangJurgen B Schnermann
Jan 27, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Laiche DjouhriSally N Lawson
Feb 25, 2006·Nature Reviews. Immunology·Carl Nathan
Mar 9, 2006·Journal of Leukocyte Biology·Heike L RittnerAlexander Brack
Jul 11, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xin FangSally N Lawson
Aug 9, 2006·Journal of Leukocyte Biology·T M Cunha, W A Verri
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Liz M LavigneJonathan S Reichner

❮ Previous
Next ❯

Citations

Jan 13, 2009·Trends in Neurosciences·Carl Y Saab, Bryan C Hains
May 20, 2008·Brain Research Reviews·Carl Y SaabBryan C Hains
Apr 19, 2011·Brain, Behavior, and Immunity·Peter M GraceMark R Hutchinson
Jul 10, 2013·The Journal of Biological Chemistry·Sangmin JeonKyoungho Suk
Jun 5, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Charlotte AllenStuart M Allan
Apr 5, 2020·Pathogens·Kathlyn Laval, Lynn W Enquist

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.