Activated T-cells and pro-inflammatory cytokines differentially regulate prostaglandin E2 secretion by mesenchymal stem cells

Biochemical and Biophysical Research Communications
Beáta HegyiFerenc Uher

Abstract

In recent years it has become clear that mesenchymal stem or stromal cells (MSCs) are capable of modulating inflammatory and immune responses through interaction with a wide variety of cells. Whereas several studies indicated that PGE2 is one of the chief soluble mediators involved in these processes, here we investigated prostaglandin E2 (PGE2) production of murine bone marrow- (BM-) and adipose tissue- (Ad-) derived MSCs stimulated with pro-inflammatory cytokines TNF-α and IFN-γ, or co-cultured with ConA-induced T-cell blasts. We found that both MSC populations are able to produce high amounts of PGE2 in MSC/activated T-cell co-cultures. This effect was markedly attenuated when direct cell-cell contact was prevented in transwell system, indicating that the elicitation of the PGE2 secretion of MSCs is contact-dependent in this experimental setting. In contrast, when soluble recombinant pro-inflammatory cytokines were added to the MSC cultures, TNF-α and IFN-γ act synergistically to induce PGE2 production, whereas only high amount of TNF-α but not IFN-γ was able to do so alone. Although the PGE2 secretion by MSCs was completely abrogated by addition of indomethacin under all culture conditions tested, L-NMA, a NOS inhibitor cou...Continue Reading

References

Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
Nov 26, 2003·Progress in Lipid Research·Makoto Murakami, Ichiro Kudo
May 11, 2006·Journal of Cell Science·Lindolfo da Silva MeirellesNance Beyer Nardi
Oct 21, 2006·Current Opinion in Hematology·Armand Keating
Aug 2, 2007·Transplantation·Aisha NasefLoïc Fouillard
Oct 13, 2007·Stem Cells·Veronika S UrbánFerenc Uher
Jan 28, 2009·Nature Reviews. Immunology·Antonio UccelliVito Pistoia
Mar 25, 2009·Experimental & Molecular Medicine·Seung-Ha YangChung-Gyu Park
Mar 9, 2010·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Ke ChenZhong Chao Han
Jun 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yoshiyasu EsakiShuh Narumiya
Nov 29, 2011·Trends in Molecular Medicine·Antje GeblerBarbara Seliger

❮ Previous
Next ❯

Citations

Oct 3, 2012·PloS One·Patricia Luz-CrawfordFarida Djouad
Jun 4, 2014·Biotechnology and Bioengineering·Jeffrey A BarminkoMartin L Yarmush
Feb 16, 2013·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Erin Collins, Gary Gilkeson
Sep 1, 2016·Experimental Cell Research·Gyöngyi KudlikFerenc Uher
Mar 26, 2016·Stem Cells Translational Medicine·Bruna CorradettiEnnio Tasciotti
Dec 5, 2016·Biochimie·Farida DjouadChristian Jorgensen
Oct 7, 2017·Frontiers in Bioengineering and Biotechnology·Bruna CorradettiEnnio Tasciotti
Aug 9, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jaleesa M GarthChad Steele
Jul 25, 2018·Stem Cells and Development·Brice MagneMarina Trouillas
May 10, 2020·Cells·Vineet Kumar MishraLiang-Yin Ke
May 30, 2019·Cancer Cell International·Mugdha SharmaSweta Srivastava
Feb 9, 2013·Expert Review of Clinical Immunology·Kevin R McIntoshJeffrey M Gimble
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Shuai ChenCunyi Fan
Jun 8, 2021·Frontiers in Bioengineering and Biotechnology·Martina SandonàValentina Saccone

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.