Activated transcription factor nuclear factor-kappa B is present in the atherosclerotic lesion

The Journal of Clinical Investigation
K BrandD Neumeier

Abstract

Nuclear factor-kappa B (NF-kappaB)/Rel transcription factors play an important role in the inducible regulation of a variety of genes involved in the inflammatory and proliferative responses of cells. The present study was designed to elucidate the implication of NF-kappaB/Rel in the pathogenesis of atherosclerosis. Activation of the dimeric NF-kappaB complex is regulated at a posttranslational level and requires the release of the inhibitor protein IkappaB. The newly developed mAb alpha-p65mAb recognizes the IkappaB binding region on the p65 (RelA) DNA binding subunit and therefore selectively reacts with p65 in activated NF-kappaB. Using immunofluorescence and immunohistochemical techniques, activated NF-kappaB was detected in the fibrotic-thickened intima/media and atheromatous areas of the atherosclerotic lesion. Activation of NF-kappaB was identified in smooth muscle cells, macrophages, and endothelial cells. Little or no activated NF-kappaB was detected in vessels lacking atherosclerosis. Electrophoretic mobility shift assays and colocalization of activated NF-kappaB with NF-kappaB target gene expression suggest functional implications for this transcription factor in the atherosclerotic lesion. This study demonstrates th...Continue Reading

References

Dec 1, 1992·The Journal of Experimental Medicine·A S NeishT Collins
Dec 1, 1991·The Journal of Clinical Investigation·J L Witztum, D Steinberg
Sep 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·E P AmentoP Libby
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S Ylä-HerttualaD Steinberg
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M P DuyaoG E Sonenshein
Mar 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J Masuda, R Ross
Nov 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A I LeveyB H Wainer
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J N WilcoxD Gordon
Oct 28, 1988·Science·P A Baeuerle, D Baltimore
Jan 1, 1995·Biological Chemistry Hoppe-Seyler·C KaltschmidtP A Baeuerle
Oct 28, 1994·Biochemical and Biophysical Research Communications·T NakajimaI Maruyama
Jan 1, 1994·Annual Review of Immunology·P A Baeuerle, T Henkel
Aug 15, 1994·Annals of the New York Academy of Sciences·B Beutler, C Van Huffel
Sep 14, 1994·Journal of Immunological Methods·P J LeenenW van Ewijk
May 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·K BrandL K Curtiss
Jan 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·R TerkeltaubL K Curtiss
Apr 1, 1993·Immunobiology·J M MüllerP A Baeuerle
May 1, 1993·The Journal of Clinical Investigation·K BrandL K Curtiss

❮ Previous
Next ❯

Citations

Jul 23, 1999·BioFactors·S R ThomasR Stocker
Jul 21, 2006·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Xiaoyun YangJun Pu
Jul 2, 2003·Neurotoxicity Research·B H Juurlink
Aug 9, 2001·Irish Journal of Medical Science·R McLaughlinD Bouchier-Hayes
Jan 12, 2007·Archives of Toxicology·Hsiu-Chung OuWen-Jane Lee
Aug 25, 2011·European Journal of Nutrition·D Zapolska-DownarD Sitkiewicz
Aug 3, 2011·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Mamiko IseUichi Ikeda
Dec 1, 2006·Cardiovascular Drugs and Therapy·Qin ZhangQiang Wang
Oct 11, 2008·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Fang LiuJun Li
Mar 3, 2006·Current Atherosclerosis Reports·Dat DoRobert Chilton
Feb 4, 2006·Current Atherosclerosis Reports·William C Aird
Apr 28, 2006·Current Atherosclerosis Reports·Neeta AdhikariJennifer L Hall
Oct 26, 2013·Current Atherosclerosis Reports·Xinghui SunMark W Feinberg
Dec 9, 2003·Trends in Molecular Medicine·Ozgur Kutuk, Huveyda Basaga
Nov 5, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Jenifer I FentonEric A Gurzell
Jul 24, 2002·Biochemical and Biophysical Research Communications·Alexandra K KiemerAngelika M Vollmar
Jan 3, 2001·European Journal of Pharmacology·O GripS Lindgren
Dec 17, 2002·Atherosclerosis·Jan Hinrich BräsenSeppo Ylä-Herttuala
Dec 31, 2003·The Journal of Surgical Research·Lauren BakerDaniel R Meldrum
Aug 29, 1998·Thrombosis Research·H DeguchiK Suzuki
Nov 14, 2002·Progress in Biophysics and Molecular Biology·Florian Wernig, Qingbo Xu
Sep 9, 2000·Molecular Aspects of Medicine·G Poli
Aug 14, 2001·Journal of the American College of Cardiology·G ValenG K Hansson
Dec 18, 2001·Free Radical Biology & Medicine·N K FukagawaB T Mossman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.