PMID: 4793Apr 1, 1976

Activation and nuclear translocation of protein kinase during transsynaptic induction of tyrosine 3-monooxygenase

Proceedings of the National Academy of Sciences of the United States of America
E CostaA Guidotti


The tyrosine-3-monooxygenase activity [L-tyrosine, tetrahydropteridine: oxygen oxidoreductase (3-hydroxylating); EC] of rat adrenal medulla is induced 20-24 hr after the injection of reserpine (16 mumol/kg intraperitoneally). This and other inducing stimuli increase the 3': 5'-cyclic AMP (cAMP) content in the medulla for longer than 60 min and activate the cAMP-dependent protein kinase (ATP: protein phosphotransferase; EC for several hours. Corticotropin (ACTH), dopamine, and propranolol do not induce the monooxygenase, but elicit an increase in the cAMP content of the medulla which fails to activate protein kinase and lasts less than 1 hr. A high- and low-molecular-weight protein kinase are separated by gel filtration from the 20,000 X g pellet extract of adrenal medulla homogenate. The activity of the low-molecular-weight enzyme is expressed as its ability to phosphorylate histone. The protein kinase activity of the pellet is increased between 3 and 17 hr after reserpine injection. Our evidence indicates that this increase is due to a translocation from cytosol to subcellular structures of a kinase that utilizes lysine-rich histone as phosphate acceptor. The protein kinase activity that is extracted from a...Continue Reading


Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S L KeelyC R Park
Sep 5, 1975·Science·G M Tomkins
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A GuidottiE Costa
Jun 20, 1975·Naunyn-Schmiedeberg's Archives of Pharmacology·U OttenH Thoenen
Dec 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·T A Langan
Dec 30, 1971·Annals of the New York Academy of Sciences·T A Langan
Feb 16, 1972·Biochemical and Biophysical Research Communications·R BalhornR Chalkley
Mar 1, 1974·Science·G S SteinL J Kleinsmith
Mar 1, 1972·Analytical Biochemistry·U Karsten, A Wollenberger
Sep 1, 1971·Journal of Neurochemistry·C Goridis, N H Neff


Nov 10, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·J P Schwartz, E Costa
Jan 1, 1981·Pharmacology & Therapeutics·V M WiegantW H Gispen
Jan 1, 1982·Progress in Neurobiology·P A KometianiE E Klein
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D L Friedman, D A Chambers
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K S KimT H Joh
Feb 1, 1982·Archives of Biochemistry and Biophysics·H C Hamman, B E Ledford
Nov 1, 1983·Experimental Cell Research·M I Mednieks, A R Hand
Apr 15, 1978·Archives of Biochemistry and Biophysics·J F KrallS G Korenman

Related Concepts

Cyclic AMP, (R)-Isomer
Adrenal Medulla
Cell Fractionation
Cell Nucleus
Enzyme Activation
Enzyme Induction
Histone H7
Protein KINASE

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