PMID: 9450948Mar 7, 1998Paper

Activation and two modes of blockade by strontium of Ca2+-activated K+ channels in goldfish saccular hair cells

The Journal of General Physiology
I Sugihara

Abstract

Effects of internal Sr2+ on the activity of large-conductance Ca2+-activated K+ channels were studied in inside-out membrane patches from goldfish saccular hair cells. Sr2+ was approximately one-fourth as potent as Ca2+ in activating these channels. Although the Hill coefficient for Sr2+ was smaller than that for Ca2+, maximum open-state probability, voltage dependence, steady state gating kinetics, and time courses of activation and deactivation of the channel were very similar under the presence of equipotent concentrations of Ca2+ and Sr2+. This suggests that voltage-dependent activation is partially independent of the ligand. Internal Sr2+ at higher concentrations (>100 microM) produced fast and slow blockade both concentration and voltage dependently. The reduction in single-channel amplitude (fast blockade) could be fitted with a modified Woodhull equation that incorporated the Hill coefficient. The dissociation constant at 0 mV, the Hill coefficient, and zd (a product of the charge of the blocking ion and the fraction of the voltage difference at the binding site from the inside) in this equation were 58-209 mM, 0.69-0.75, 0.45-0.51, respectively (n = 4). Long shut events (slow blockade) produced by Sr2+ lasted approxima...Continue Reading

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Citations

Mar 2, 2005·The Journal of General Physiology·Xu-Hui ZengChristopher J Lingle
Jul 27, 2005·The Journal of General Physiology·Tinatin I Brelidze, Karl L Magleby
Sep 1, 2005·The Journal of General Physiology·Manoj N KrishnanEdward Moczydlowski
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·S BianE Moczydlowski
Aug 15, 2008·Journal of Neurophysiology·Adrian Rodriguez-ContrerasEbenezer N Yamoah
Sep 25, 2012·Bioorganicheskaia khimiia·L A LysenkoN N Nemova
Nov 28, 2014·Journal of Neurophysiology·Consuelo Morgado-ValleLuis Beltran-Parrazal
Mar 11, 2020·Annual Review of Physical Chemistry·Mangesh I ChaudhariSusan B Rempe
Jun 17, 2018·The Journal of Chemical Physics·Mangesh I Chaudhari, Susan B Rempe

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