PMID: 9557923Apr 29, 1998Paper

Activation by calcium of erythrocyte Na+/H+ exchange in men

Journal of Hypertension
P LijnenV Petrov

Abstract

To determine whether protein kinase C is necessary for the calcium activation of the Na+/H+ exchange in human erythrocytes by studying activation by calcium of erythrocyte Na+/H+ exchange in control cells, in protein kinase C-depleted cells after downregulation of protein kinase C with phorbol-12-myristate-13-acetate and in cells that had been treated beforehand with phorbol-12-myristate-13-acetate with and without the calpain inhibitor E-64d. Erythrocyte Na+/H+ exchange was measured by determining the initial rates of the influx of Na+ into Na+-depleted, acid loaded cells. The effects of various concentrations (0-1 mmol/l) of CaCl2 and the effects of 1 mmol/l CaCl2 on activation of the intracellular pH and on the external Na+ activation of Na+/H+ exchange were studied. The effects of 1 mmol/l CaCl2 on Na+/H+ exchange in control cells and cells that had been incubated beforehand with and without 1 micromol/l phorbol-12-myristate-13-acetate and with E-64d and 1 micromol/l phorbol-12-myristate-13-acetate for 1, 2, 3 and 24 h were also investigated. Addition of Ca2+ to a concentration in the range 0-1 mmol/l in the presence of calcimycin resulted in stimulation of Na+/H+ exchange: 1 mmol/l CaCl2 increased (P< 0.001) the erythrocyt...Continue Reading

References

Apr 1, 1989·Physiological Reviews·E K Hoffmann, L O Simonsen
Mar 1, 1989·The Journal of Membrane Biology·A SempliciniM Canessa
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H E Ives, T O Daniel
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Oct 1, 1995·Hypertension·W Siffert, R Düsing
Sep 1, 1993·The Biochemical Journal·S E WilkinsonJ S Nixon
May 1, 1993·Hypertension·D RosskopfW Siffert
Apr 15, 1993·The Biochemical Journal·H Hug, T F Sarre
Aug 1, 1995·Journal of Hypertension·P Lijnen, V Petrov

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