Activation kinetics and single channel properties of recombinant alpha1beta2gamma2L GABA(A) receptor channels

Neuroreport
K JahnC Franke

Abstract

Recombinant alpha1beta2gamma2L GABA(A) receptor channels, transiently expressed in HEK 293 cells, were investigated using the patch-clamp technique in combination with a device for ultra-fast solution exchange. The dose-response relationship revealed an EC50 of 11.6 +/- 0.9 microM and saturated with 3 mM GABA. The slope between 0.001 and 0.01 mM GABA was 2.2 +/- 0.4, indicating at least three binding sites for GABA. The rise time decreased from about 120 ms at 0.001 mM GABA to about 0.8 ms at 10 mM GABA. Single channel openings were grouped in bursts with an average duration of 10.3 +/- 3.0 ms. More than 95% of the current was represented by a single channel slope conductance of about 29 pS.

References

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Citations

May 7, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·K JahnK Krampfl
Apr 15, 2005·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Bahram MohammadiJohannes Bufler
Apr 20, 2000·European Journal of Pharmacology·H AdelsbergerJ Dudel
Mar 29, 2001·European Journal of Pharmacology·B MohammadiJ Bufler
May 24, 2003·Neuropharmacology·A J BoileauR A Pearce
Oct 25, 2003·Journal of Neuropathology and Experimental Neurology·Susanne PetriJohannes Bufler

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