PMID: 6967568Jul 31, 1980Paper

'Activation-labile' glucocorticoid-receptor complexes of a steroid-resistant variant of CEM-C7 human lymphoid cells

Nature
T J SchmidtE B Thompson

Abstract

For cytoplasmic glucocorticoid-receptor complexes to enter and accumulate in the nucleus a temperature-dependent event, 'activation' is required. Activation can be achieved in vitro by increased ionic strength, dilution or gel filtration and is manifested by an increased affinity of steroid-receptor complex for DNA and an altered elution profile from ion-exchange resins. Munck and Foley have shown that activated complexes isolated from thymocytes elute from DEAE-cellulose in a manner identical to complexes activated in vitro. We report here that DEAE-cellulose chromatography of steroid-receptor complexes from CEM-C7, a cloned human leukaemic T-cell line sensitive to the cytolytic action of glucocorticoids, and its steroid-resistant subclone 4R4 demonstrated that steroid receptors of clone 4R4 cannot form stable activated complexes. This defines a new defect in receptor action, activation lability (r+act1), which is unlike either the r-, r+nt-, or r+nti phenotypes previously described for mouse lymphoid variants.

References

Feb 1, 1979·Journal of Cellular Physiology·J M HarmonE B Thompson
Jul 1, 1979·Journal of Steroid Biochemistry·D Toft, H Nishigori
Apr 1, 1978·Molecular and Cellular Endocrinology·M PfahlS Bourgeois
May 17, 1977·Biochemistry·J A GoidlG Litwack
Jul 25, 1977·Biochemical and Biophysical Research Communications·Y Sakaue, E B Thompson
Oct 1, 1977·Archives of Biochemistry and Biophysics·L G Parchman, G Litwack
Sep 25, 1973·Journal of Molecular Biology·G G RousseauG M Tomkins
Dec 4, 1973·Biochemistry·E MilgromE E Baulieu
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·K R YamamotoG M Tomkins
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J D BaxterG M Tomkins

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Citations

Aug 1, 1985·Klinische Wochenschrift·E B ThompsonJ M Harmon
Mar 1, 1981·Journal of Steroid Biochemistry·J M HarmonE B Thompson
Jun 1, 1982·Journal of Steroid Biochemistry·T W HutchensF S Markland
May 1, 1982·Journal of Steroid Biochemistry·E F HawkinsF S Markland
Jul 1, 1983·Journal of Steroid Biochemistry·G P ChrousosM B Lipsett
Jan 1, 1984·Journal of Steroid Biochemistry·N J HolbrookA Munck
Jan 1, 1987·Journal of Steroid Biochemistry·E B Thompson
Mar 1, 1990·Journal of Steroid Biochemistry·J P CarboneR L Brent
Jan 1, 1983·Molecular and Cellular Endocrinology·R M GledhillM R Norman
Dec 1, 1986·Molecular and Cellular Endocrinology·U Gehring
Sep 4, 1981·Biochimica Et Biophysica Acta·N Murakami, V K Moudgil
Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·E B ThompsonB H Johnson
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S S Simons, E B Thompson
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G A PucaA M Molinari
Jun 1, 1982·The Journal of Clinical Investigation·G P ChrousosM B Lipsett
Apr 1, 1981·Environmental Health Perspectives·E B Thompson
Apr 1, 1993·In Vitro Cellular & Developmental Biology. Animal·S KawaE B Thompson
Apr 1, 1994·Leukemia & Lymphoma·G J KaspersA J Veerman
Jan 1, 1984·Leukemia Research·R BellR McCaffrey
Jan 1, 1983·Leukemia Research·C H PuiG V Dahl
Oct 16, 1989·Biochemical and Biophysical Research Communications·P R Housley, A M Forsthoefel
Mar 1, 1982·Archives of Biochemistry and Biophysics·N MurakamiV K Moudgil
Jul 8, 2008·Acta Biochimica Et Biophysica Sinica·E Brad Thompson
Feb 1, 1986·The American Journal of Physiology·G M ShacklefordL J Berry
Jun 1, 1987·Journal of Steroid Biochemistry·C E RekerW V Vedeckis

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