Abstract
P19 embryonic carcinoma cells, a model system for studying early development and differentiation, can differentiate into neurons and primitive endoderm-like cells depending on the culture conditions. We have previously reported that the activation of c-Jun amino-terminal kinase (JNK) is required for the retinoic acid-induced neural differentiation of P19 cells. However, the signaling pathway(s) responsible for the activation of JNK has not been known. In this study, we demonstrated that activities of MAPK kinase 4 (MKK4) and TAK1, one of the upstream kinases of MKK4, were enhanced in the neurally differentiating cells. Inhibition of the neural differentiation by an overexpression of protein phosphatase 2Cepsilon, an inactivator of TAK1, suggested a critical role of the TAK1 signaling pathway during the differentiation. Confocal microscopic analysis indicated that TAK1, phospho-MKK4, and phospho-JNK were colocalized with tubulin in the neurites and localized also in the nuclei of the differentiating cells. In contrast, two TAK1-binding proteins, TAB1 and TAB2, which are involved in the activation of TAK1, were localized in the neurites and the nuclei of the differentiating cells, respectively. These results suggest that two dist...Continue Reading
References
Jul 1, 1983·Developmental Biology·M K Edwards, M W McBurney
Dec 9, 1994·Science·A MindenM Karin
Dec 22, 1994·Nature·I SánchezL I Zon
Dec 22, 1994·Nature·M YanD J Templeton
May 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G BainD I Gottlieb
Dec 22, 1995·Science·K YamaguchiK Matsumoto
Oct 18, 1996·The Journal of Biological Chemistry·Y SasaharaS Tamura
Nov 29, 1996·The Journal of Biological Chemistry·B I Giasson, W E Mushynski
Jan 3, 1997·Science·H IchijoY Gotoh
Mar 28, 1997·The Journal of Biological Chemistry·P GerwinsG L Johnson
May 30, 1997·The Journal of Biological Chemistry·K Deacon, J L Blank
Jun 13, 1997·The Journal of Biological Chemistry·S i HiraiS Ohno
Jul 1, 1997·Journal of Neurochemistry·C H ReynoldsB H Anderton
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·C TournierR J Davis
Sep 26, 1997·The Journal of Biological Chemistry·E H Jho, C C Malbon
Sep 26, 1997·The Journal of Biological Chemistry·E H JhoC C Malbon
Nov 14, 1997·The Journal of Biological Chemistry·H SakumaT Kawasaki
Jan 31, 1998·The EMBO Journal·T MoriguchiE Nishida
Mar 27, 1999·Nature·J Ninomiya-TsujiK Matsumoto
Apr 21, 1999·Current Opinion in Cell Biology·T P Garrington, G L Johnson
Mar 4, 2000·The Journal of Biological Chemistry·K KishimotoJ Ninomiya-Tsuji
Apr 12, 2000·Progress in Neurobiology·K Mielke, T Herdegen
May 16, 2000·The Journal of Biological Chemistry·J KanungoH y Wang
Nov 1, 2000·Cell·R J Davis
Mar 22, 2001·Molecular and Cellular Biology·G TakaesuK Matsumoto
Aug 22, 2001·FEBS Letters·H WangS Tamura
Aug 24, 2001·The Journal of Biological Chemistry·Y QianX Li
Nov 9, 2001·The Journal of Biological Chemistry·Hsien-yu WangCraig C Malbon
Jan 16, 2002·Current Opinion in Genetics & Development·Claire R Weston, Roger J Davis
Aug 2, 2002·Cell·Sung Hee BaekMichael G Rosenfeld
Jan 31, 2003·The Journal of Biological Chemistry·Ming Guang LiShinri Tamura
Mar 26, 2003·FEBS Letters·Tada-aki KudoTakayasu Kobayashi
Citations
Nov 1, 2011·Journal of Biochemistry·Taeko MiyagiShinri Tamura
Nov 30, 2005·Molecular and Cellular Biology·Claudia R AmuraLynn E Heasley
Aug 6, 2005·Endocrinology·Nedal SafwatWilliam L Miller
Sep 19, 2012·Neuroscience·O Gonzalez-PerezJ M Garcia-Verdugo
Feb 5, 2015·The Journal of Veterinary Medical Science·Yuka MaruyamaKatsuhiko Arai
May 29, 2007·Experimental Eye Research·I A MaltsevaC Basbaum
Aug 28, 2009·Cell Biochemistry and Function·Kohei NakayaMasaru Murakami
Jun 19, 2007·Differentiation; Research in Biological Diversity·Caihong XiaNaihe Jing
Jan 30, 2007·Developmental Biology·D A Rappolee
May 14, 2016·Scientific Reports·Guan WangJianhua Zhao
Jul 19, 2016·American Journal of Human Genetics·Emma M WadeStephen P Robertson
Mar 19, 2005·Carcinogenesis·Yongliang ZhuSuzhang Zhang
Nov 11, 2008·The Journal of Biological Chemistry·Baukje M ElzingaJustin V McCarthy
Mar 23, 2017·Neurochemical Research·Lu LuShiqing Feng
Jul 21, 2021·Biochemical and Biophysical Research Communications·Ikuko KakizakiKeiichi Takagaki