Activation mechanism of recombinant Der p 3 allergen zymogen: contribution of cysteine protease Der p 1 and effect of propeptide glycosylation.

The Journal of Biological Chemistry
Marie-Eve DumezAndy Chevigné

Abstract

The trypsin-like protease Der p 3, a major allergen of the house dust mite Dermatophagoides pteronyssinus, is synthesized as a zymogen, termed proDer p 3. No recombinant source of Der p 3 has been described yet, and the zymogen maturation mechanism remains to be elucidated. The Der p 3 zymogen was produced in Pichia pastoris. We demonstrated that the recombinant zymogen is glycosylated at the level of its propeptide. We showed that the activation mechanism of proDer p 3 is intermolecular and is mediated by the house dust mite cysteine protease Der p 1. The primary structure of the proDer p 3 propeptide is associated with a unique zymogen activation mechanism, which is different from those described for the trypsin-like family and relies on the house dust mite papain-like protease Der p 1. This is the first report of a recombinant source of Der p 3, with the same enzymatic activity as the natural enzyme and trypsin. Glycosylation of the propeptide was found to decrease the rate of maturation. Finally, we showed that recombinant Der p 3 is inhibited by the free modified prosequence T(P1)R.

References

Dec 1, 1975·Biochemical Pharmacology·S Cha
Jul 15, 1989·Lancet·G A StewartR J Simpson
Dec 1, 1987·The Journal of Allergy and Clinical Immunology·T A Platts-Mills, M D Chapman
Apr 20, 1967·Biochemical and Biophysical Research Communications·I Schechter, A Berger
Mar 1, 1994·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W A SmithW R Thomas
May 1, 1993·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H YasuedaH Yamakawa
Feb 1, 1996·International Archives of Allergy and Immunology·W A Smith, W R Thomas
Oct 1, 1996·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B J Bennett, W R Thomas
Aug 1, 1997·The Journal of Allergy and Clinical Immunology·K MaruoH Maeda
Dec 22, 1999·Progress in Biophysics and Molecular Biology·J L Arrondo, F M Goñi
Sep 21, 2000·The Journal of Clinical Investigation·W HalangkJ Deussing
Jul 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·G SunS Mattoli
Jul 9, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A JacquetA Bollen
Oct 10, 2002·International Archives of Allergy and Immunology·Wayne R ThomasRichard M O'Brien
Apr 24, 2003·Allergy·N CheongK Y Chua
Jul 2, 2003·Molecular Biology and Evolution·Jian-Min ChenMiklós Sahin-Tóth
Jan 28, 2006·International Archives of Allergy and Immunology·Toshiro TakaiHideoki Ogawa
Aug 8, 2006·The Journal of Allergy and Clinical Immunology·Belinda J HalesWayne R Thomas
Oct 6, 2007·Journal of Molecular Biology·Andy ChevignéMoreno Galleni
Oct 6, 2009·The Journal of Biological Chemistry·Eva Kereszturi, Miklós Sahin-Tóth

❮ Previous
Next ❯

Citations

Jun 3, 2009·PLoS Neglected Tropical Diseases·Jan DvorákConor R Caffrey
Aug 5, 2010·Biochimica Et Biophysica Acta·Andy ChevignéMoreno Galleni
Nov 20, 2014·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Ahlem BouazizMarie-Eve Dumez
Apr 20, 2014·Frontiers in Immunology·Marie-Eve DumezAndy Chevigné
Aug 19, 2016·Current Allergy and Asthma Reports·Emmanuel NonyPhilippe Moingeon
Nov 30, 2016·International Journal of Molecular Sciences·Peter Goettig
May 1, 2017·International Archives of Allergy and Immunology·Eduardo Santos da SilvaFatima Ferreira
Nov 13, 2014·Molecular & Cellular Proteomics : MCP·Adnan HalimHans H Wandall
Jun 28, 2017·International Journal of Molecular Sciences·Manuel Reithofer, Beatrice Jahn-Schmid

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.