Activation of 5-HT4 receptors facilitates neurogenesis from transplanted neural stem cells in the anastomotic ileum

The Journal of Physiological Sciences : JPS
Kei GotoMiyako Takaki

Abstract

An orally administered serotonin-4 (5-HT4) receptor agonist, mosapride citrate (MOS), promotes enteric neurogenesis in anastomoses after gut surgery. We performed gut surgery and transplanted 2 × 10(5) neural stem cells (NSCs) from the embryonic central nervous system after marking them with the cell linker, PKH26. We found that neurons differentiated from transplanted NSCs (PKH [+]) and newborn enteric neurons differentiated from mobilized (host) NSCs (YFP [+]) in the deep granulation tissue of the anastomotic ileum. MOS significantly increased the number of PKH (+) and YFP (+) neurons by 2.5-fold (P < 0.005) (n = 4). The distribution patterns of PKH (+) neurons and YFP (+) neurons were similar along the depth of the anastomosis. A 5-HT4 receptor antagonist, SB-207266, abolished these effects of MOS (n = 4). Our results indicate that neurogenesis from transplanted NSCs is potentiated by activation of 5-HT4 receptors. Thus, a combination of drug administration and cell transplantation could be more beneficial than cell transplantation alone in treating Hirschsprung's disease and related disorders.

References

Jun 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Masaya YamanouchiMiyako Takaki
Mar 24, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Abdelhak BelmadaniRichard J Miller
Dec 29, 2007·Immunological Investigations·Paul K Wallace, Katharine A Muirhead
Mar 1, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Renta KatsuiMiyako Takaki
Apr 3, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hiroaki WakeJunichi Nabekura
Aug 7, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Min-Tsai LiuMichael D Gershon
Feb 12, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·H MatsuyoshiM Takaki
Feb 22, 2011·Biochemical and Biophysical Research Communications·Miyako TakakiHiroki Kuniyasu
Dec 24, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Isao KawaharaMiyako Takaki
Aug 21, 2012·Nature Neuroscience·Chihiro NishiyamaHideki Enomoto
Aug 24, 2012·Stem Cells and Development·Peng LiXiaodan Jiang
Aug 16, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Quan FindlayLincon A Stamp

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Citations

Feb 3, 2016·Frontiers in Cellular Neuroscience·Francisco J RiveraLudwig Aigner
Jul 28, 2016·Trends in Neurosciences·Kara Gross Margolis, Michael D Gershon
Mar 4, 2017·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·F GiancolaR Chiocchetti
Jan 5, 2018·Nature Reviews. Gastroenterology & Hepatology·Robert O Heuckeroth
Jun 10, 2020·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Yuying ZhaoYa Gao
Jul 27, 2018·Nature Reviews. Neuroscience·Meenakshi Rao, Michael D Gershon
Dec 15, 2015·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Miyako TakakiJunich Nabekura

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Methods Mentioned

BETA
fluorescence
confocal microscopy
fluorescence microscopy
transgenic

Software Mentioned

Image J

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