Activation of a helper and not regulatory human CD4+ T cell response by oncolytic H-1 parvovirus.

PloS One
Olivier MoralèsNadira Delhem

Abstract

H-1 parvovirus (H-1 PV), a rodent autonomous oncolytic parvovirus, has emerged as a novel class of promising anticancer agents, because of its ability to selectively find and destroy malignant cells. However, to probe H-1 PV multimodal antitumor potential one of the major prerequisites is to decipher H-1 PV direct interplay with human immune system, and so prevent any risk of impairment. Non activated peripheral blood mononuclear cells (PBMCs) are not sensitive to H-1 PV cytotoxic effect. However, the virus impairs both activated PBMC proliferation ability and viability. This effect is related to H-1 PV infection as evidenced by Western blotting detection of H-1 PV main protein NS1. However, TCID50 experiments did not allow newly generated virions to be detected. Moreover, flow cytometry has shown that H-1 PV preferentially targets B lymphocytes. Despite seeming harmful at first sight, H-1 PV seems to affect very few NK cells and CD8+ T lymphocytes and, above all, clearly does not affect human neutrophils and one of the major CD4+ T lymphocyte subpopulation. Very interestingly, flow cytometry analysis and ELISA assays proved that it even activates human CD4+ T cells by increasing activation marker expression (CD69 and CD30) and...Continue Reading

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Citations

Apr 5, 2015·Current Opinion in Virology·Karsten GeletnekyJean Rommelaere
May 8, 2019·Viruses·Assia Angelova, Jean Rommelaere
Aug 24, 2019·Frontiers in Immunology·Antonio MarchiniJean Rommelaere
Aug 29, 2021·Cancer Gene Therapy·David Mealiea, J Andrea McCart

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Methods Mentioned

BETA
flow cytometry
Enzyme-Linked ImmunoSorbent Assay
ELISA
density gradient centrifugation
Magnetic-Activated Cell-Sorting
light microscopy
transfection
Protein Assay
electrophoresis

Software Mentioned

MikroWin 2000
Ascent
Sigma
EXPO
Windows Multiple Document Interface for Flow Cytometry Softwar...
stat

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