PMID: 6539228Jan 1, 1984Paper

Activation of acetylcholine esterase (ACHE) as a sign for erythrocyte membrane alteration

Experimental Pathology
K J HalbhuberW Linss

Abstract

In vitro activity determination of membrane bound acetylcholine esterase (ACHE) of intact erythrocytes and differently altered RBC forms by means of the ELLMAN method yielded increase in activity in erythrocyte/vesicle mixtures and ghosts. Alteration of the cells by treatment with periodate, neuraminidase and trypsin leads to no, weak or clear decrease in the activity of this enzyme. Old cells also show a clear decrease in activity. When cells were treated with diamide ACHE is at first functionally inhibited, finally, however, it reaches supernormal values of activity. Possible causes for the different behaviour of ACHE in dependence on the degree of alteration of the erythrocytes are discussed. The results of the measurements were compared with the ultrahistochemical ACHE reactions according to KARNOVSKY (thiocholine--copper ferricyanide method) and IWAYAMA (lead thiocholine method). The influence of several components of the KARNOVSKY'S histochemical reaction mixture on ACHE activity was tested. The investigations suggest that disintegration of the erythrocyte membrane is accompanied with activation of the ACHE molecules which are nearly inactive in the intact membrane. This activation contributes to the positive histochemica...Continue Reading

References

Dec 15, 1979·Experientia·H GrossmannM Liefländer
May 4, 1978·Biochimica Et Biophysica Acta·C W HaestB Deuticke
Jul 28, 1977·Nature·R F ZwaalL L van Deenen
Jan 17, 1972·Biochimica Et Biophysica Acta·M Heller, D J Hanahan
Mar 29, 1974·Biochimica Et Biophysica Acta·B Aloni, A Livne
May 14, 1972·Journal of Molecular Biology·T L Steck
Dec 1, 1966·Okajimas Folia Anatomica Japonica·T Iwayama
Jan 1, 1981·Progress in Biophysics and Molecular Biology·P J Quinn
Jan 1, 1982·Haemostasis·R F Zwaal, H C Hemker
Feb 23, 1982·Biochimica Et Biophysica Acta·K H FischbeckD L Schotland
Jan 8, 1981·Biochimica Et Biophysica Acta·C Di Francesco, U Brodbeck
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE

❮ Previous
Next ❯

Citations

Apr 1, 1984·The Histochemical Journal·K J HalbhuberJ Makovitzky
Jan 1, 1988·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Korpela, H Tähti
Mar 4, 2000·Life Sciences·Y G PrallF R Ampy
Aug 1, 1988·American Journal of Hematology·M Koekebakker, R D Barr
Sep 1, 1987·American Journal of Hematology·A A Lawson, R D Barr

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.