PMID: 15383576Sep 24, 2004Paper

Activation of acid sphingomyelinase and its inhibition by the nitric oxide/cyclic guanosine 3',5'-monophosphate pathway: key events in Escherichia coli-elicited apoptosis of dendritic cells

The Journal of Immunology : Official Journal of the American Association of Immunologists
Sestina FalconeEmilio Clementi

Abstract

Depletion of dendritic cells (DCs) via apoptosis contributes to sepsis-induced immune suppression. The mechanisms leading to DC apoptosis during sepsis are not known. In this study we report that immature DCs undergo apoptosis when treated with high numbers of Escherichia coli. This effect was mimicked by high concentrations of LPS. Apoptosis was accompanied by generation of ceramide through activation of acid sphingomyelinase (A-SMase), was prevented by inhibitors of this enzyme, and was restored by exogenous ceramide. Compared with immature DCs, mature DCs expressed significantly reduced levels of A-SMase, did not generate ceramide in response to E. coli or LPS, and were insensitive to E. coli- and LPS-triggered apoptosis. However, sensitivity to apoptosis was restored by addition of exogenous A-SMase or ceramide. Furthermore, inhibition of A-SMase activation and ceramide generation was found to be the mechanism through which the immune-modulating messenger NO protects immature DCs from the apoptogenic effects of E. coli and LPS. NO acted through formation of cGMP and stimulation of the cGMP-dependent protein kinase. The relevance of A-SMase and its inhibition by NO/cGMP were confirmed in a mouse model of LPS-induced sepsis. ...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D M GadboisE M Bradbury
Jan 1, 1991·Chest·R L DannerJ E Parillo
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·V E LaubachP A Sherman
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·J HambletonA L DeFranco
Sep 12, 1997·The Journal of Biological Chemistry·C ScioratiE Clementi
Jan 7, 1998·The Journal of Experimental Medicine·A Haimovitz-FriedmanR Kolesnick
Apr 1, 1998·Nature·J Banchereau, R M Steinman
Apr 29, 1998·Annual Review of Physiology·R N Kolesnick, M Krönke
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·E ClementiS Moncada
Jun 24, 1998·The Journal of Trauma·J C PuyanaC L Miller
Apr 27, 1999·Biochimica Et Biophysica Acta·T Levade, J P Jaffrézou
Nov 11, 1999·Cell Death and Differentiation·L Liu, J S Stamler
Jan 15, 2000·The European Journal of Surgery = Acta Chirurgica·M DitschkowskiH Grosse-Wilde
Mar 18, 2000·The Journal of Biological Chemistry·T LinD R Green
May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·C De NadaiE Clementi
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M MuzioA Mantovani
Jun 14, 2000·American Journal of Respiratory and Critical Care Medicine·J L VincentJ C Preiser
Jun 27, 2000·The Journal of Biological Chemistry·S KirschnekE Gulbins
Mar 23, 2001·Nature Immunology·M Moser, K M Murphy
Nov 30, 2000·The Journal of Biological Chemistry·F ParisR Kolesnick
Mar 22, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H P DeignerR Kinscherf
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C OberholzerL L Moldawer
May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R S HotchkissI E Karl
Jul 17, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·R DhamiE H Schuchman
Aug 18, 2001·Cell·J BanchereauG Schuler
Sep 11, 2001·Journal of the American College of Surgeons·J A MannickJ A Lederer
Sep 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T KantoA A Amoscato
Sep 29, 2001·Nature Immunology·C Bogdan
Nov 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M M MonickG W Hunninghake

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Citations

Jan 5, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Juan J LasarteClaude Leclerc
Mar 27, 2007·Surgical Infections·Joseph CuschieriRonald V Maier
Dec 13, 2006·Expert Opinion on Investigational Drugs·Silvia BrunelliEmilio Clementi
Sep 12, 2008·Biological Chemistry·Heike GrassméErich Gulbins
Mar 21, 2009·The EMBO Journal·Fabio BiancoClaudia Verderio
Jun 23, 2010·Apoptosis : an International Journal on Programmed Cell Death·Ekaterina ShumilinaFlorian Lang
Jul 13, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Veera Venkata Ratnam BandaruNorman J Haughey
Dec 24, 2010·Journal of Leukocyte Biology·Daniela GiordanoEdward A Clark
Dec 21, 2005·Angewandte Chemie·Veit Wascholowski, Athanassios Giannis
Oct 1, 2009·Shock·Carl F FortinOlivier Lesur
Sep 9, 2011·Therapeutic Advances in Respiratory Disease·Yang Yang, Stefan Uhlig
Jun 22, 2014·The Journal of Biological Chemistry·David M PerryYusuf A Hannun
Dec 19, 2017·Frontiers in Physiology·Sandra TorresJose C Fernandez-Checa
Dec 21, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Daniela S KempeFlorian Lang
May 3, 2011·Cellular and Molecular Life Sciences : CMLS·Jean-Philip TrumanSamar M Hammad
Sep 12, 2008·Biological Chemistry·Cristiana PerrottaEmilio Clementi
Sep 18, 2014·Frontiers in Physiology·Nadine BeckmannBärbel Edelmann
Mar 31, 2015·BioMed Research International·Xia FanHua-ping Liang
Mar 10, 2017·Antioxidants & Redox Signaling·Yuqing WuHeike Grassmé
Oct 28, 2017·Journal of Immunology Research·Dong-Dong WuXin-Ying Ji
Oct 3, 2018·Surgical Infections·Jennifer E BakerMichael J Edwards
Dec 27, 2018·Cellular Microbiology·Alberto Fernández-OlivaCristina Risco
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rimpei MoritaToshiyuki Hori
Oct 20, 2010·The Journal of Biological Chemistry·Cristiana PerrottaEmilio Clementi
Jun 21, 2018·Biological Chemistry·Yuqing WuHeike Grassmé
May 5, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carlos Ocaña-MorgnerRolf Jessberger
May 6, 2019·Journal of Biophotonics·Muhannad N FadhelMichael C Kolios
May 1, 2021·International Journal of Molecular Sciences·François AncienMarianne Rooman

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