Activation of alpha-protein kinase C leads to association with detergent-insoluble components of GH4C1 cells

Molecular Endocrinology
S C Kiley, S Jaken

Abstract

TRH and phorbol dibutyrate (PDBu) stimulate PRL secretion and synthesis from GH4C1 rat pituitary cells through activation of protein kinase C (PKC). TRH responses are mediated by increases in cellular levels of two PKC activators, Ca2+ and diacylglycerol (DAG), whereas PDBu acts as a DAG analog. We conducted experiments to compare the effects of Ca2+ and PDBu/DAG on alpha-PKC redistribution and to determine to what components of the particulate fraction activated alpha-PKC associates. Subcellular fractionation experiments demonstrated that TRH and PDBu both caused chelator-stable association of alpha-PKC with the particulate fraction. In contrast, Ca2+-mediated association with the particulate fraction was not chelator stable. Immunocytofluorescence experiments also demonstrated that TRH, PDBu, and increased cytosolic Ca2+ (due to ionomycin or K+ depolarization) caused redistribution. The effect of TRH was rapid and transient, similar to TRH stimulation of phospholipase C. The translocated alpha-PKC in the particulate fraction from TRH- or PDBu-treated cultures was not solubilized with Triton X-100. In comparable studies using an immunofluorescence assay, alpha-PKC immunofluorescence remained in detergent-insoluble preparations...Continue Reading

Citations

Feb 1, 1991·Journal of Cellular Physiology·E M RosenI D Goldberg
Jan 1, 1991·Pharmacology & Therapeutics·S Stabel, P J Parker
Jun 9, 1998·Cellular Signalling·C Keenan, D Kelleher
Nov 24, 1999·The Biochemical Journal·E Rendón-HuertaM Robles-Flores
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D Mochly-RosenJ Lopez
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·D RonD Mochly-Rosen
Aug 1, 1990·Cell Regulation·D Mochly-RosenP C Simpson
Feb 5, 2002·Annual Review of Physiology·Gerald W Dorn, Daria Mochly-Rosen
Jan 17, 1997·The Journal of Biological Chemistry·M YedovitzkyR Nesher
Oct 28, 2003·The Journal of Biological Chemistry·Swaminathan MurugappanSatya P Kunapuli
Feb 1, 1994·Circulation Research·V O Rybin, S F Steinberg
Oct 25, 2006·Journal of Thrombosis and Haemostasis : JTH·B N KahnerS P Kunapuli
Sep 29, 2011·FEBS Letters·Sagit CohenNoah Isakov
Oct 4, 1996·The Journal of Biological Chemistry·J A JohnsonD Mochly-Rosen
Dec 21, 2002·The Journal of Biological Chemistry·Daniel K GreeneAnne Woods
Jan 1, 2004·Biological Reviews of the Cambridge Philosophical Society·Thomas W Pike, Marion Petrie
May 1, 1995·Biological Reviews of the Cambridge Philosophical Society·S Krackow
May 12, 1998·The American Journal of Physiology·J A MaloneyJ R Williamson
Oct 1, 1990·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Gary Freeman, Ellis B Ridgway
Jun 1, 1997·Protein Expression and Purification·M Robles-FloresJ A García-Sáinz
Aug 21, 1996·Biochimica Et Biophysica Acta·C GodsonP A Insel
Nov 16, 1992·Biochemical and Biophysical Research Communications·B L Smith, D Mochly-Rosen
Apr 1, 1990·Current Opinion in Cell Biology·S Jaken

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.