Activation of AMP-activated protein kinase by adiponectin rescues salivary gland epithelial cells from spontaneous and interferon-gamma-induced apoptosis

Arthritis and Rheumatism
Stergios KatsiougiannisFotini N Skopouli

Abstract

Primary Sjögren's syndrome (SS) is an autoimmune disease characterized by lymphocytic infiltrates associated with destruction of salivary gland epithelial cells (SGECs) induced mainly by apoptosis. Adiponectin is an immunoregulatory hormone. We have previously shown that SGECs from patients with primary SS as well as from controls differentially express adiponectin. SGECs derived from patients with primary SS constitutively produce and secrete adiponectin in higher quantities. The aim of this study was to investigate the effect of adiponectin on the proliferation and apoptosis of SGECs. Cultured, non-neoplastic SGECs were treated with recombinant human adiponectin, and the rate of cell proliferation was assessed. Spontaneous and interferon-gamma (IFNgamma)-induced apoptosis was evaluated with a specific single-stranded DNA enzyme-linked immunosorbent assay. The AMP-activated protein kinase (AMPK) inhibitor Compound C was used to test the involvement of AMPK in adiponectin effects. Western blotting was applied to detect the phosphorylation levels of AMPK after adiponectin treatment. Adiponectin treatment resulted in a dose-dependent suppression of proliferation of SGECs from patients with primary SS and control donors. Adiponect...Continue Reading

Citations

May 16, 2012·European Journal of Nutrition·Robert KrysiakBoguslaw Okopien
Sep 10, 2013·Experimental Eye Research·Stephen C PflugfelderCintia S de Paiva
Sep 28, 2010·American Journal of Respiratory Cell and Molecular Biology·Ming ZhuStephanie A Shore
Jan 11, 2011·Arthritis Research & Therapy·Ewa A SzyszkoKathrine Skarstein
Apr 18, 2014·Journal of Immunology Research·Vanessa AbellaOreste Gualillo
Dec 14, 2011·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Brian AstryKamal D Moudgil
May 28, 2019·Chemical Senses·Sean M CrossonSergei Zolotukhin

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