Activation of AMPK improves lipopolysaccharide-induced dysfunction of the blood-brain barrier in mice

Brain Injury : [BI]
Hai-Ya YuZhan Liu

Abstract

Lipopolysaccharide (LPS) is known to alter the integrity of the blood-brain barrier (BBB) in sepsis, although the underlying mechanism remains unknown. The aim of this study was to elucidate the molecular mechanisms underlying disruption of the BBB in LPS-induced sepsis. Both in vitro and in vivo experiments were designed to test the role of AMP-activated protein kinase (AMPK) in LPS-induced BBB dysfunction. Human brain microvascular endothelial cells (HBMECs) were cultured. The protein expressions were detected by western blot. BBB integrity was determined by Evans Blue. LPS (1 μg ml(-1)) dramatically increased the permeability of the BBB and the ROS productions, as well as reducing the expression levels of occludin and claudin-5 in cultured HBMECs. Inhibition of NAD(P)H oxidase by apocynin or up-regulation of AMPK reversed the LPS-induced abnormities in HBMECs. In LPS-induced sepsis in mice, it was found that LPS dramatically increased NAD(P)H oxidase protein expressions and ROS productions in the brain and disrupted BBB function assayed by Evans blue staining, which were abolished by AICAR treatment. It is concluded that AMPK activation improves the functions of the BBB impaired by LPS through suppression of NAD(P)H oxidase-...Continue Reading

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Citations

Mar 21, 2016·Brain, Behavior, and Immunity·Aravinthan Varatharaj, Ian Galea
Mar 11, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Min Hu, Bo Liu
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Jun 26, 2018·Frontiers in Neuroscience·Md Mamun Al-AminHasan Mahmud Reza
Dec 16, 2020·Tissue Barriers·Tatiana BarichelloFelipe Dal-Pizzol
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Yue HaoXianhong Gu

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