Activation of antioxidant pathways in ras-mediated oncogenic transformation of human surface ovarian epithelial cells revealed by functional proteomics and mass spectrometry

Cancer Research
Travis W YoungXiaodong Cheng

Abstract

Cellular transformation is a complex process involving genetic alterations associated with multiple signaling pathways. Development of a transformation model using defined genetic elements has provided an opportunity to elucidate the role of oncogenes and tumor suppressor genes in the initiation and development of ovarian cancer. To study the cellular and molecular mechanisms of Ras-mediated oncogenic transformation of ovarian epithelial cells, we used a proteomic approach involving two-dimensional electrophoresis and mass spectrometry to profile two ovarian epithelial cell lines, one immortalized with SV40 T/t antigens and the human catalytic subunit of telomerase and the other transformed with an additional oncogenic ras(V12) allele. Of approximately 2200 observed protein spots, we have identified >30 protein targets that showed significant changes between the immortalized and transformed cell lines using peptide mass fingerprinting. Among these identified targets, one most notable group of proteins altered significantly consists of enzymes involved in cellular redox balance. Detailed analysis of these protein targets suggests that activation of Ras-signaling pathways increases the threshold of reactive oxidative species (ROS...Continue Reading

References

May 1, 1991·Journal of the National Cancer Institute·S B LiT L Yang-Feng
Sep 1, 1990·Gynecologic Oncology·V V BakerD Miller
Oct 1, 1984·In Vitro·N AuerspergS E Myrdal
Jan 16, 1995·FEBS Letters·S ToyokuniH Hiai
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Mar 19, 1997·Journal of the National Cancer Institute·M WanL Dubeau
Jul 20, 1999·Experimental and Molecular Pathology·Z R WangS R Young
Aug 10, 1999·Nature·W C HahnR A Weinberg
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Feb 24, 2001·Antioxidants & Redox Signaling·A Miranda-VizueteG Spyrou
May 26, 2001·Annual Review of Nutrition·D Behne, A Kyriakopoulos
Sep 22, 2001·Human Pathology·Y HashiguchiS Ogita
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Diane R WonseyChi V Dang
Mar 20, 2003·Journal of the National Cancer Institute·Gad SingerIe-Ming Shih
Aug 2, 2003·Gynecologic Oncology·Mary L GemignaniJeff Boyd
Mar 5, 2004·Cancer Research·Jinsong LiuRobert C Bast

❮ Previous
Next ❯

Citations

Oct 16, 2004·Current Oncology Reports·Wei HuJohn J Kavanagh
Oct 7, 2011·Cell Death & Disease·D R Gough, T G Cotter
May 30, 2009·Nature Reviews. Drug Discovery·Dunyaporn TrachoothamPeng Huang
Sep 22, 2006·Antioxidants & Redox Signaling·Toren Finkel
Jun 5, 2008·Antioxidants & Redox Signaling·Dunyaporn TrachoothamPeng Huang
Nov 17, 2012·Molecular Biology of the Cell·Aarthi Ravichandran, Boon Chuan Low
May 27, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Daniel A SymondsJodi A Flaws
Mar 7, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·A P G CrijnsE G E de Vries
Jul 4, 2012·Reproductive Biology and Endocrinology : RB&E·Ashok AgarwalSajal Gupta
Jul 16, 2005·Reproductive Biology and Endocrinology : RB&E·Ashok AgarwalRakesh K Sharma
Dec 9, 2004·Pharmacogenomics·David E MisekSamir M Hanash
Mar 28, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Yoke Keong YongZuraini Ahmad
Aug 21, 2012·Therapeutic Delivery·Xiaohua Peng, Varsha Gandhi
Dec 3, 2013·The Journal of Clinical Investigation·Andrea GlasauerNavdeep S Chandel
Jun 22, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Jun-Ping Liu, He Li
Apr 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Arvind RamanathanStuart L Schreiber
May 1, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jana JeschkeNita Ahuja
Nov 19, 2013·Current Opinion in Oncology·Shang-Jui Wang, Wei Gu
May 28, 2011·Free Radical Research·Mareike KelkelMarc Diederich
Feb 18, 2016·Oxidative Medicine and Cellular Longevity·Aysegul CortLiudmila Korkina
Apr 2, 2011·Science China. Life Sciences·GuoQing LiZhuChu Chen
Dec 10, 2009·International Journal of Cancer. Journal International Du Cancer·Shira Yaari-StarkYoel Kloog
Jun 1, 2006·Drug Discovery Today. Disease Models·Sanja Sale, Sandra Orsulic
Feb 7, 2006·Free Radical Biology & Medicine·Manuela Gago-Dominguez, J Esteban Castelao
Mar 22, 2014·Biochemical Society Transactions·Naila RabbaniPaul J Thornalley
Jul 2, 2008·Drug Discovery Today. Disease Models·Weiqin LuPeng Huang
Dec 11, 2013·Free Radical Biology & Medicine·Francesca R ŠalipurPaula J Bates
Feb 15, 2011·Seminars in Cell & Developmental Biology·Paul J Thornalley, Naila Rabbani
Aug 1, 2014·Biochemical Pharmacology·Andrea Glasauer, Navdeep S Chandel
Nov 18, 2006·Molecular & Cellular Proteomics : MCP·Travis W YoungXiaodong Cheng
Feb 3, 2007·Journal of Cellular Biochemistry·Gerald C ChuRonald A DePinho
Jan 11, 2007·Proteomics·Christian V RecktenwaldBarbara Seliger
Feb 12, 2005·Human Reproduction Update·Mahmoud R Hussein

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis