Activation of beta-globin promoter by erythroid Krüppel-like factor.

Molecular and Cellular Biology
H Asano, G Stamatoyannopoulos

Abstract

Erythroid Krüppel-like factor (EKLF), an erythroid tissue-specific Krüppel-type zinc finger protein, binds to the beta-globin gene CACCC box and is essential for beta-globin gene expression. EKLF does not activate the gamma gene, the CACCC sequence of which differs from that of the beta gene. To test whether the CACCC box sequence difference is the primary determinant of the selective activation of the beta gene by EKLF, the CACCC boxes of beta and gamma genes were swapped and the resulting promoter activities were assayed by transient transfections in CV-1 cells. EKLF activated the beta promoter carrying a gamma CACCC box at a level comparable to that at which it activated the wild-type beta promoter, whereas EKLF failed to activate a gamma promoter carrying the beta CACCC box, despite the presence of the optimal EKLF binding site. Similar results were obtained in K562 cells. The possibility that overexpressed EKLF superactivated the beta promoter carrying the gamma CACCC box, or that EKLF activated the mutated beta promoter through the intact distal CACCC box, was excluded. To test whether the position of the CACCC box in the beta or gamma promoter determined EKLF specificity, the proximal beta CACCC box sequence was created ...Continue Reading

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Citations

Jul 10, 2001·The Journal of Biological Chemistry·J J Bieker
Oct 22, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Xin-Jun JiChih-chuan Liang
Apr 17, 1999·Molecular and Cellular Biology·H AsanoG Stamatoyannopoulos
Apr 5, 2001·Molecular and Cellular Biology·X Chen, J J Bieker
Nov 16, 2019·Scientific Reports·Natasha MalikAlison M Michie
Aug 2, 2001·Hemoglobin·M H Steinberg
Mar 29, 2002·Pediatric Research·Antonio Cao, Paolo Moi
Apr 16, 2005·Molecular and Cellular Biology·Akane OmoriKeiji Tanimoto
Sep 4, 2004·The Biochemical Journal·Haruhiko AsanoGeorge Stamatoyannopoulos
Dec 19, 2001·The Journal of Biological Chemistry·Chao-Zhong SongGeorge Stamatoyannopoulos
Feb 13, 2001·Blood Cells, Molecules & Diseases·Y YangG Stamatoyannopoulos
Oct 12, 2000·Mechanisms of Development·A Kawahara, I B Dawid
Aug 5, 1998·Current Opinion in Genetics & Development·R A Swank, G Stamatoyannopoulos
Feb 18, 2005·Biochimica Et Biophysica Acta·Ayako MitsumaTomoki Naoe

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