PMID: 8610116Apr 2, 1996Paper

Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages

Proceedings of the National Academy of Sciences of the United States of America
J HambletonA L DeFranco

Abstract

Activation of macrophages by bacterial lipopolysaccharide (LPS) induces transcription of genes that encode for proinflammatory regulators of the immune response. Previous work has suggested that activation of the transcription factor activator protein 1 (AP-1) is one LPS-induced event that mediates this response. Consistent with this notion, we found that LPS stimulated AP-1-mediated transcription of a transfected reporter gene in the murine macrophage cell line RAW 264.7. As AP-1 activity is regulated in part by activation of the c-Jun N-terminal kinase (JNK), which phosphorylates and subsequently increases the transcriptional activity of c-Jun, we examined whether LPS treatment of macrophages resulted in activation of this kinase. LPS treatment of RAW 264.7 cells, murine bone marrow-derived macrophages, and the human monocyte cell line THP-1 resulted in rapid activation of the p46 and p54 isoforms of JNK. Treatment with wild-type and rough mutant forms of LPS and synthetic lipid A resulted in JNK activation, while pretreatment with the tyrosine kinase inhibitor herbimycin A inhibited this response. Binding of LPS-LPS binding protein (LBP) complexes to CD14, a surface receptor that mediates many LPS responses, was found to be ...Continue Reading

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Citations

Apr 22, 2008·Inflammatory Bowel Diseases·Yea Ping LinJames Versalovic
Oct 11, 2002·The Journal of Pathology·Koichiro YoshidaNobuyuki Hara
Sep 18, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yeo Myeong LeeJung Han Yoon Park
Dec 28, 2007·Glycoconjugate Journal·Feng-Ling YangShih-Hsiung Wu
Nov 29, 2012·Molecular Biology Reports·Styliani TakaNicholas P Anagnou
Aug 28, 2010·Journal of Natural Medicines·Gi-Sang BaeSung-Joo Park
Jun 4, 1999·Progress in Biophysics and Molecular Biology·M H Cobb
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Aug 7, 2002·Alcohol·Craig J McClainShirish Barve
Mar 21, 2001·Cellular Signalling·M Guha, N Mackman
Feb 28, 2004·Trends in Cardiovascular Medicine·L New, J Han
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Sep 30, 2000·Immunology·H D Brightbill, R L Modlin
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Sep 8, 2007·Antioxidants & Redox Signaling·Alexander HoetzelStefan W Ryter

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