Activation of C-reactive protein proinflammatory phenotype in the blood retinal barrier in vitro: implications for age-related macular degeneration.

Aging
Sara Romero-VázquezBlanca Molins

Abstract

The retinal pigment epithelium (RPE) is considered one of the main targets of age-related macular degeneration (AMD), the leading cause of irreversible vision loss among the ageing population worldwide. Persistent low grade inflammation and oxidative stress eventually lead to RPE dysfunction and disruption of the outer blood-retinal barrier (oBRB). Increased levels of circulating pentameric C-reactive protein (pCRP) are associated with higher risk of AMD. The monomeric form (mCRP) has been detected in drusen, the hallmark deposits associated with AMD, and we have found that mCRP induces oBRB disruption. However, it is unknown how mCRP is generated in the subretinal space. Using a Transwell model we found that both pCRP and mCRP can cross choroidal endothelial cells and reach the RPE in vitro and that mCRP, but not pCRP, is able to cross the RPE monolayer in ARPE-19 cells. Alternatively, mCRP can originate from the dissociation of pCRP in the surface of lipopolysaccharide-damaged RPE in both ARPE-19 and primary porcine RPE lines. In addition, we found that the proinflammatory phenotype of mCRP in the RPE depends on its topological localization. Together, our findings further support mCRP contribution to AMD progression enhancing...Continue Reading

References

Sep 5, 2001·Molecular Immunology·J E Volanakis
Aug 29, 2002·The Journal of Biological Chemistry·Tarek KhreissJános G Filep
Sep 5, 2002·American Journal of Ophthalmology·Don H AndersonLincoln V Johnson
Oct 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·John W CrabbJoe G Hollyfield
Apr 14, 2004·Archives of Ophthalmology·Marco A Zarbin
Sep 1, 2004·The Journal of Biological Chemistry·Steven BlackDavid Samols
Mar 12, 2005·Science·Jonathan L HainesMargaret A Pericak-Vance
Mar 12, 2005·Science·Albert O EdwardsLindsay A Farrer
Sep 20, 2005·The British Journal of Ophthalmology·A C BrowningW M Amoaku
Oct 28, 2005·American Journal of Physiology. Cell Physiology·Rosalia C MoraEnrique Rodriguez-Boulan
Feb 28, 2006·Survey of Ophthalmology·Larry A DonosoGregory Hageman
Apr 8, 2006·Stroke; a Journal of Cerebral Circulation·Jerzy KrupinskiLina Badimon
Nov 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·P T JohnsonL V Johnson
Nov 23, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shang-Rong JiJing Zhao
Jul 7, 2007·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·George BoguslawskiCarlos A Labarrere
Sep 13, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Blanca MolinsLina Badimon
Jan 13, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shang-Rong JiYi Wu
Feb 23, 2010·Journal of Atherosclerosis and Thrombosis·Christopher SchneeweisStephan Goetze
Jun 3, 2011·The British Journal of Ophthalmology·Imran A BhuttoGerard A Lutty
Sep 21, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nadine LauerPeter F Zipfel
Oct 1, 2011·Investigative Ophthalmology & Visual Science·Zsolt AblonczyCraig E Crosson
May 25, 2013·Nature Reviews. Immunology·Jayakrishna AmbatiBradley D Gelfand
Oct 26, 2013·Nature Reviews. Immunology·Albert C ShawRuth R Montgomery
Jul 5, 2016·The Journal of Pathology·Kathleen R ChircoRobert F Mullins
Dec 23, 2016·American Journal of Physiology. Cell Physiology·Blanca MolinsJordi Martorell
Mar 30, 2018·Frontiers in Immunology·Lin ZhangYi Wu
May 5, 2018·Frontiers in Immunology·Blanca MolinsAndrew D Dick
Oct 24, 2018·Progress in Retinal and Eye Research·Mei ChenHeping Xu
Dec 6, 2018·International Journal of Molecular Sciences·Effat AlizadehHelder André

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Citations

Jul 23, 2021·Ophthalmology·Mark P BreazzanoUNKNOWN American Academy of Ophthalmology

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Methods Mentioned

BETA
ELISA
protein assay
electrophoresis

Software Mentioned

ImageJ
GraphPad
GraphPad Prism

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