Abstract
1. Ionic currents were simultaneously recorded at macroscopic and unitary level using the whole-cell and cell-attached patch-clamp procedures together on the same portion of isolated mouse skeletal muscle fibres. 2. In the presence of Tyrode solution in the patch pipette and Tyrode-TTX solution in the bath, macroscopic and unitary currents through delayed rectifier K+ channels were simultaneously recorded in response to depolarizing pulses of 1 s duration. 3. In five fibres, successive long-lasting incremental depolarizing levels induced, at -40 mV or -30 mV, the opening of a high conductance channel carrying an outward current superimposed on delayed rectifier K+ channel activity. Opening of this high conductance channel was not observed when the depolarization steps were applied in the patch pipette. 4. Using the same depolarizing protocol, activation of a high conductance channel was also observed in two fibres in the presence of a K+-rich solution in the pipette (145 mM K+) . 5. With either Tyrode or K+-rich solution in the pipette, unitary current amplitudes of the high conductance channel matched well with the values obtained for Ca2+-activated K+ (KCa) channels in inside-out patches under similar ionic conditions. 6. Ind...Continue Reading
References
May 1, 1978·The Journal of Physiology·A Duval, C Léoty
Sep 1, 1991·The Journal of General Physiology·H Westerblad, D G Allen
Nov 1, 1991·Pflügers Archiv : European journal of physiology·H BrinkmeierR Rüdel
Aug 1, 1991·Journal of Bioenergetics and Biomembranes·O B McManus
May 1, 1990·The Journal of Physiology·F L Burton, O F Hutter
Nov 1, 1989·Trends in Neurosciences·A Marty
Jan 1, 1989·Annual Review of Physiology·R LatorreO Alvarez
Jul 1, 1974·The Journal of Cell Biology·T L SprayJ R Sommer
Oct 1, 1982·The Journal of Physiology·J N BarrettB S Pallotta
Apr 1, 1983·The Journal of General Physiology·K G Beam, P L Donaldson
May 15, 1996·The Journal of Physiology·S D Hocherman, F Bezanilla
Jun 1, 1996·Journal of Bioenergetics and Biomembranes·G J KaczorowskiM L Garcia
Jul 15, 1996·The Journal of Physiology·B AllardV Jacquemond
Aug 1, 1997·Biophysical Journal·V Jacquemond
Sep 1, 1960·The Journal of Physiology·A L HODGKIN, P HOROWICZ
Citations
Sep 23, 2009·Acta Physiologica·M Kristensen, C Juel
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·N MalloukB Allard
Oct 17, 2002·The Journal of Physiology·Maëlle JospinBruno Allard
May 23, 2002·Biophysical Journal·Nora Mallouk, Bruno Allard
Jul 2, 2008·The Journal of Physiology·S P Cairns, M I Lindinger
Jun 17, 2006·The Journal of Physiology·Bruno AllardVincent Jacquemond
Oct 15, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Michael KristensenCarsten Juel
Mar 11, 2000·American Journal of Physiology. Cell Physiology·N Mallouk, B Allard
Aug 15, 2019·American Journal of Physiology. Cell Physiology·Katja K PedersenKristian Overgaard
Jan 13, 2015·Journal of Applied Physiology·Simeon P CairnsJean-Marc Renaud
Oct 4, 2000·Physiological Reviews·O M Sejersted, G Sjøgaard
Mar 29, 2000·Biophysical Journal·A E OliverJ H Crowe
Apr 8, 2009·Biophysical Journal·Céline Berbey, Bruno Allard