Activation of Ca2+-activated K+ channels by an increase in intracellular Ca2+ induced by depolarization of mouse skeletal muscle fibres

The Journal of Physiology
V Jacquemond, Bruno Allard

Abstract

1. Ionic currents were simultaneously recorded at macroscopic and unitary level using the whole-cell and cell-attached patch-clamp procedures together on the same portion of isolated mouse skeletal muscle fibres. 2. In the presence of Tyrode solution in the patch pipette and Tyrode-TTX solution in the bath, macroscopic and unitary currents through delayed rectifier K+ channels were simultaneously recorded in response to depolarizing pulses of 1 s duration. 3. In five fibres, successive long-lasting incremental depolarizing levels induced, at -40 mV or -30 mV, the opening of a high conductance channel carrying an outward current superimposed on delayed rectifier K+ channel activity. Opening of this high conductance channel was not observed when the depolarization steps were applied in the patch pipette. 4. Using the same depolarizing protocol, activation of a high conductance channel was also observed in two fibres in the presence of a K+-rich solution in the pipette (145 mM K+) . 5. With either Tyrode or K+-rich solution in the pipette, unitary current amplitudes of the high conductance channel matched well with the values obtained for Ca2+-activated K+ (KCa) channels in inside-out patches under similar ionic conditions. 6. Ind...Continue Reading

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Citations

Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·N MalloukB Allard
Jul 2, 2008·The Journal of Physiology·S P Cairns, M I Lindinger
Oct 15, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Michael KristensenCarsten Juel
Mar 11, 2000·American Journal of Physiology. Cell Physiology·N Mallouk, B Allard
Aug 15, 2019·American Journal of Physiology. Cell Physiology·Katja K PedersenKristian Overgaard
Oct 4, 2000·Physiological Reviews·O M Sejersted, G Sjøgaard
Mar 29, 2000·Biophysical Journal·A E OliverJ H Crowe

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