Activation of Ca2+-dependent currents in dorsal root ganglion neurons by metabotropic glutamate receptors and cyclic ADP-ribose precursors

Journal of Neurophysiology
J H CrawfordR H Scott

Abstract

Cultured dorsal root ganglion neurons were voltage clamped at -90 mV to study the effects of intracellular application of nicotinamide adenine dinucleotide (betaNAD+), intracellular flash photolysis of caged 3',5'-cyclic guanosine monophosphate (cGMP), and metabotropic glutamate receptor activation. The activation of metabotropic glutamate receptors evoked inward Ca2+-dependent currents in most cells. This was mimicked both by intracellular flash photolysis of the caged axial isomer of cGMP [P-1-(2-nitrophenyl)ethyl cGMP] and intracellular application of betaNAD+. Whole cell Ca2+-activated inward currents were used as a physiological index of raised intracellular Ca2+ levels. Extracellular application of 10 microM glutamate evoked the activation of Ca2+-dependent inward currents, thus reflecting a rise in intracellular Ca2+ levels. Similar inward currents were also activated after isolation of metabotropic glutamate receptor activation by application of 10 microM glutamate in the presence of 20 microM 6-cyano-7-nitroquinoxaline-2,3-dione and 20 microM dizocilpine maleate (MK 801), or by extracellular application of 10 microM trans-(1S,3R)-1-amino-1,3-cyclopentanedicarboxylic acid. Intracellular photorelease of cGMP, from its ca...Continue Reading

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Citations

Feb 1, 2002·FEBS Letters·Andrew J LokutaHéctor H Valdivia
Jul 12, 2013·PloS One·Ling-Hsuan KungLuc Jasmin
Oct 30, 2019·Cells·Nicholas E Karagas, Kartik Venkatachalam
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May 27, 2015·Biochemical Society Transactions·Aidan Kaar, Mark G Rae
Jan 15, 2010·Journal of Neurophysiology·Charlene M HickeyNeil S Magoski
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Feb 5, 2000·Progress in Neurobiology·S FringsS J Kleene
Aug 1, 2007·Neurochemistry International·Haruhiro HigashidaMami Noda

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