Activation of camalexin biosynthesis in Arabidopsis thaliana in response to perception of bacterial lipopolysaccharides: a gene-to-metabolite study.

Planta
Caryn Ann BeetsIan A Dubery

Abstract

Lipopolysaccharides (LPS), as lipoglycan microbe-associated molecular pattern molecules, trigger activation of signal transduction pathways involved in defence that generate an enhanced defensive capacity in plants. The transcriptional regulation of the genes for tryptophan synthase B, TSB1, and the cytochrome P450 monooxygenases CYP79B2 and CYP71B15, involved in the camalexin biosynthetic pathway, were investigated in response to LPS treatment. GUS-reporter assays for CYP71B15 and CYP79B2 gene promoter activation were performed on transgenic plants and showed positive histochemical staining in response to LPS treatment, indicating activation of the promoters. Quantitative PCR revealed that transcripts of TSB1, CYP79B2 and CYP71B15 exhibited differential, transient up-regulation. TSB1 transcript levels were up-regulated between 6 and 9 h after LPS-induction, while CYP71B15 and CYP79B2 both exhibited maxima at 12 h. To obtain information on the gene-to-metabolite network, the effect of the transcriptome changes on the metabolome was correlated to camalexin production. Increases in camalexin concentration were quantified by ultra pressure liquid chromatography-mass spectrometry and both absorbance spectra and elemental compositio...Continue Reading

References

Jul 1, 1995·The Plant Cell·E R Radwanski, R L Last
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·J Glazebrook, F M Ausubel
Nov 1, 1996·Molecular Plant-microbe Interactions : MPMI·E E RogersF M Ausubel
Mar 6, 1999·Current Opinion in Plant Biology·P J Rushton, I E Somssich
Sep 4, 1999·The Plant Journal : for Cell and Molecular Biology·B P ThommaW F Broekaert
Feb 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·A K HullJ L Celenza
Apr 29, 2000·Trends in Plant Science·T EulgemI E Somssich
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·S C van WeesC M Pieterse
Nov 4, 2000·Nature Biotechnology·O FiehnL Willmitzer
Jan 3, 2001·The Plant Journal : for Cell and Molecular Biology·H SakaiA Oka
Nov 10, 2001·Annual Review of Phytopathology·Ray Hammerschmidt
Aug 17, 2002·Current Opinion in Plant Biology·Karam SinghLuis Oñate-Sánchez
Apr 17, 2004·The Plant Journal : for Cell and Molecular Biology·Katherine J DenbyDaniel J Kliebenstein
Apr 28, 2004·Trends in Biotechnology·Royston GoodacreDouglas B Kell
May 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Erich GlawischnigBarbara Ann Halkier
Oct 23, 2004·Proceedings of the National Academy of Sciences of the United States of America·Dana ZeidlerJörg Durner
Aug 5, 2005·Annual Review of Phytopathology·Jane Glazebrook
Sep 20, 2005·The Plant Journal : for Cell and Molecular Biology·Daniel J KliebensteinKatherine J Denby
Nov 29, 2005·Journal of Bioscience and Bioengineering·Eiichiro Fukusaki, Akio Kobayashi
Mar 1, 2006·BMC Bioinformatics·Joshua S YuanC Neal Stewart
Apr 29, 2006·Biochemical and Biophysical Research Communications·Natasha M Sanabria, Ian A Dubery
Jun 13, 2006·Plant Physiology·Regina SchuheggerErich Glawischnig
Jun 14, 2006·Journal of Plant Physiology·Regina SchuheggerErich Glawischnig
Aug 8, 2006·Plant Physiology and Biochemistry : PPB·I B GerberI A Dubery
Jan 16, 2007·Phytochemistry·Erich Glawischnig
Jul 11, 2007·Journal of Endotoxin Research·Mari-Anne NewmanMichelangelo Parrilli
May 10, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Elia GrataSerge Rudaz
Jul 1, 2008·Current Opinion in Plant Biology·Saskia C M Van WeesCorné M J Pieterse
Jan 22, 2009·The Plant Journal : for Cell and Molecular Biology·Francesca L StefanatoHenk-jan Schoonbeek

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Citations

Apr 11, 2013·Journal of the American Chemical Society·Dorianne A CastilloSeiichi P T Matsuda
Jul 11, 2014·Frontiers in Plant Science·Adam L HeubergerJessica E Prenni

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