PMID: 11929953Apr 4, 2002Paper

Activation of cancer-specific gene expression by the survivin promoter

Journal of the National Cancer Institute
R BaoThomas C Hamilton

Abstract

Survivin, a member of the IAP (inhibitor of apoptosis) gene family, appears to be overexpressed in common cancers but not in corresponding normal adult tissues. To investigate whether the survivin promoter controls cancer cell-specific gene expression, we determined whether the survivin gene promoter could regulate reporter gene expression in cancer cell lines and xenografts. Survivin protein levels were determined in human and murine cancer cell lines and in normal tissues of adult C57BL/6 mice by Western blot analysis. A reporter construct in which a portion of the survivin gene promoter was used to drive transcription of a human secreted alkaline phosphatase (SEAP) gene was transiently transfected into cancer cells, and promoter activity was extrapolated from SEAP activity. A2780 human ovarian cancer cells were transfected with this construct, and stable transfectants were injected into the intrabursal ovarian space of immunodeficient mice. Tumor growth was monitored, and plasma SEAP levels were used as a measure of survivin promoter activity in vivo. Survivin protein was detected in all cancer cell lines examined but not in most normal adult mouse tissues. After transfection, the survivin promoter was more active in all can...Continue Reading

Citations

Jun 7, 2005·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Shi LuZehua Wang
Jul 16, 2011·Hormones & Cancer·Antonio F SaadAnil K Sood
Dec 14, 2007·Nature Reviews. Cancer·Dario C Altieri
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rochelle R ArvizoPriyabrata Mukherjee
Sep 18, 2013·Cancer Biotherapy & Radiopharmaceuticals·Yanyun WangJin Si
Aug 13, 2009·Molecular Cancer Therapeutics·Xiaoming XieMien-Chie Hung
Dec 24, 2010·Journal of Visualized Experiments : JoVE·Selene Nunez-CruzNathalie Scholler
Mar 13, 2012·Molecular Biotechnology·Salim S El-AmouriDao Pan
Oct 2, 2007·FEBS Letters·Klaus W SommerChrista Cerni
Jan 29, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Anjaiah SrirangamDavid A Potter
Dec 29, 2011·Seminars in Cancer Biology·Benjamin Sredni
Jun 30, 2009·Cancer Treatment Reviews·Bríd M RyanMichael J Duffy
Mar 10, 2016·Pharmaceutical Research·Robert RiehleVladimir Torchilin
Jun 10, 2008·Cancer Science·Hirofumi YamamotoMorito Monden
Jul 29, 2011·Journal of Cellular and Molecular Medicine·Xin-Ran LiuXin-Yuan Liu
Oct 18, 2005·Gynecologic Oncology·Parameshwar J MahasreshtiDavid T Curiel
Oct 8, 2008·Cellular & Molecular Biology Letters·Krystyna KonopkaNejat Düzgüneş
Jan 16, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Charles N LandenAnil K Sood
Jul 6, 2010·Chinese Medical Sciences Journal = Chung-kuo I Hsüeh K'o Hsüeh Tsa Chih·Feng ChenChih-chuan Liang
Sep 16, 2004·The Journal of Gene Medicine·Daniel T ReinDavid T Curiel
Aug 20, 2005·Cancer Gene Therapy·I KazhdanR A Marciniak
Feb 23, 2007·Leukemia·M RomagnoliS Barillé-Nion
Jul 30, 2003·Journal of Dental Research·C TanakaH Tanzawa
May 27, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zui-Su YangGuo-Fang Ding
Mar 16, 2004·Cancer Gene Therapy·Zeng B ZhuDavid T Curiel
Dec 27, 2017·Journal of Cellular Physiology·Luni EmdadPaul B Fisher
Jun 24, 2004·Journal of Cellular Biochemistry·Dario C Altieri
Sep 19, 2015·Cancer Growth and Metastasis·Alexander S BobbsKaren D Cowden Dahl
Dec 17, 2005·Antioxidants & Redox Signaling·Amit K GargBharat B Aggarwal
Aug 28, 2003·Journal of Cellular Physiology·Fengzhi Li
Apr 20, 2006·Journal of Neurosurgery·Winan J Van HoudtZeng B Zhu
Nov 15, 2011·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Bei ZhangJunnian Zheng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.