Activation of caspase-3 in the skeletal muscle during haemodialysis.

European Journal of Clinical Investigation
M A BoivinD S C Raj

Abstract

Muscle atrophy in end-stage renal disease (ESRD) may be due to the activation of apoptotic and proteolytic pathways. We hypothesized that activation of caspase-3 in the skeletal muscle mediates apoptosis and proteolysis during haemodialysis (HD). Eight ESRD patients were studied before (pre-HD) and during HD and the findings were compared with those from six healthy volunteers. Protein kinetics was determined by primed constant infusion of L-(ring (13)C(6) ) Phenylalanine. Caspase-3 activity in the skeletal muscle was higher in ESRD patients pre-HD than in controls (24966·0 ± 4023·9 vs. 15293·3 ± 2120·0 units, P<0·01) and increased further during HD (end-HD) (37666·6 ± 4208·3 units) (P<0·001). Actin fragments (14 kDa) generated by caspase-3 mediated cleavage of actomyosin was higher in the skeletal muscle pre-HD (68%) and during HD (164%) compared with controls. The abundance of ubiquitinized carboxy-terminal actin fragment was also significantly increased during HD. Skeletal muscle biopsies obtained at the end of HD exhibited augmented apoptosis, which was higher than that observed in pre-HD and control samples (P<0·001). IL-6 content in the soluble fraction of the muscle skeletal muscle was increased significantly during HD. ...Continue Reading

References

Apr 1, 1992·Acta Physiologica Scandinavica·A Holmäng, P Björntorp
Jan 1, 1991·The Journal of Clinical Investigation·G Z FaddaS G Massry
Dec 1, 1987·The American Journal of Physiology·L L MoldawerK G Lundholm
Nov 1, 1971·Clinical Science·L Jorfeldt, J Wahren
Jul 15, 1994·Annals of Internal Medicine·S KapadiaD L Mann
Feb 1, 1994·Proceedings of the Society for Experimental Biology and Medicine·M N Goodman
May 21, 1993·Cell·B M Spiegelman, G S Hotamisligil
Aug 1, 1997·The American Journal of Physiology·D L AllenV R Edgerton
Aug 1, 1997·Intensive Care Medicine·M Brockhaus
Dec 10, 1997·Journal of the National Cancer Institute·M J Tisdale
Apr 16, 2002·British Journal of Cancer·N CarbóJ M Argilés
Aug 21, 2002·American Journal of Respiratory and Critical Care Medicine·Alvar G N AgustíXavier Busquets
Mar 13, 2003·Kidney International·Steven M HarwoodMuhammad M Yaqoob
Jul 15, 2003·American Journal of Physiology. Endocrinology and Metabolism·Adam SteensbergBente Klarlund Pedersen
Dec 5, 2003·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Dominic S C RajPhilip G Zager
Jan 1, 2004·The Journal of Clinical Investigation·Jie DuWilliam E Mitch
May 22, 2004·Journal of the American Society of Nephrology : JASN·Seoung Woo LeeWilliam E Mitch
May 3, 2005·The Journal of Clinical Investigation·Kathryn E Wellen, Gökhan S Hotamisligil
Jun 25, 2005·Sports Medicine·Amie J Dirks, Christiaan Leeuwenburgh
Aug 5, 2005·Oncogene·Sophie LaunayCarmen Garrido
Jun 2, 2006·Journal of the American Society of Nephrology : JASN·Stewart H LeckerWilliam E Mitch
Sep 29, 2006·Journal of the American Society of Nephrology : JASN·Biruh T WorkenehWilliam E Mitch
Feb 1, 2007·American Journal of Physiology. Endocrinology and Metabolism·Dominic S C RajArny Ferrando
Aug 11, 2007·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Sílvia BusquetsJosep M Argilés
Nov 22, 2007·European Journal of Applied Physiology·S KoçtürkE Ozer
Nov 27, 2007·European Journal of Clinical Investigation·D S C RajP Moseley
Feb 22, 2008·Kidney International·D S C RajA Ferrando
Jun 10, 2008·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Juan Jesús CarreroAbdul Rashid Qureshi

❮ Previous
Next ❯

Citations

Aug 24, 2012·American Journal of Physiology. Renal Physiology·Wai W Cheung, Robert H Mak
Oct 22, 2011·Journal of Applied Physiology·Michael I LewisJoel D Kopple
Aug 20, 2011·Clinical Journal of the American Society of Nephrology : CJASN·Ilia BeberashviliJoshua Weissgarten
Apr 30, 2013·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ibrahim H Fahal
May 8, 2013·Journal of Renal Nutrition : the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation·Cynthia DelgadoKirsten L Johansen
Apr 9, 2011·Journal of Cachexia, Sarcopenia and Muscle·Robert H MakKamyar Kalantar-Zadeh
May 14, 2011·Hemodialysis International·Hussein KassamAntonios H Tzamaloukas
Mar 6, 2015·Journal of Muscle Research and Cell Motility·Benjamin T BarnesEsther E Dupont-Versteegden
Feb 23, 2013·Journal of Renal Nutrition : the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation·Juan Jesús CarreroHarold A Franch
Feb 18, 2014·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·Elizabeth J Carey
Nov 24, 2011·Current Opinion in Clinical Nutrition and Metabolic Care·Giacomo GaribottoDaniela Verzola
Mar 8, 2017·Nutrients·Yan Zha, Qi Qian
Sep 29, 2012·American Journal of Physiology. Renal Physiology·Maria R Wing, Dominic S Raj
May 10, 2020·Journal of Renal Nutrition : the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation·Yae Lim LeeSang Yoon Lee
Aug 31, 2021·Frontiers in Nutrition·Giacomo GaribottoGiuliano Brunori

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

American Journal of Physiology. Endocrinology and Metabolism
D S C RajArny Ferrando
Current Opinion in Nephrology and Hypertension
Dominic S C RajA H Tzamaloukas
Journal of the American Society of Nephrology : JASN
Liping ZhangWilliam E Mitch
© 2021 Meta ULC. All rights reserved