Activation of caspase-9 is required for UV-induced apoptosis of human keratinocytes.

The Journal of Biological Chemistry
Leonid A SitailoMitchell F Denning

Abstract

UV radiation from the sun activates both the membrane death receptor and the intrinsic or mitochondrial apoptotic signaling pathways in epidermal keratinocytes, triggering apoptosis and affording protection against skin cancer formation. We have investigated the involvement of caspase-9 in the UV death effector pathway in human keratinocytes, since this is the initiating caspase in the mitochondrial pathway required for UV-induced apoptosis in some, but not all, cell types. UV radiation triggered activation of caspase-3, caspase-9, and caspase-8 with similar kinetics, although the rank order of activation was caspase-3 > caspase-9 > caspase-8. Inhibition of caspase-9 with either the peptide inhibitor benzyloxycarbonyl-Leu-Glu(OCH(3))-His-Asp(OCH(3))-fluoromethyl ketone, or expression of a catalytically inactive caspase-9 by retroviral transduction, protected normal keratinocytes from UV-induced apoptosis. HaCaT keratinocytes harboring mutant p53 alleles were also protected from UV-induced apoptosis by the dominant negative caspase-9. The dominant negative caspase-9 blocked UV-induced activation of caspase-3, caspase-9, and caspase-8, and also protected cells from the loss of mitochondrial membrane potential. In contrast, the do...Continue Reading

References

Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Dec 22, 1994·Nature·A ZieglerD E Brash
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A ZieglerA E Bale
May 1, 1993·Carcinogenesis·T A LehmanE M Rogan
Aug 1, 1996·Human Gene Therapy·T M Kinsella, G P Nolan
Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·K H Kraemer
May 1, 1997·Experimental Cell Research·M LeverkusB A Gilchrest
Nov 5, 1997·The Journal of Biological Chemistry·A RehemtullaV M Dixit
Aug 15, 1997·The Biochemical Journal·G M Cohen
Jan 10, 1998·The Journal of Investigative Dermatology·G LiV Ho
Apr 16, 1998·The Journal of Investigative Dermatology·M LeverkusB A Gilchrest
Jun 20, 1998·The Journal of Biological Chemistry·T KuwanaS Kornbluth
Aug 28, 1998·Science·A Ashkenazi, V M Dixit
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Aug 28, 1998·Science·D R Green, J C Reed
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·H O FearnheadY A Lazebnik
Feb 13, 1999·The Journal of Biological Chemistry·X M SunG M Cohen
Mar 20, 1999·The Journal of Biological Chemistry·H R StennickeG S Salvesen
Apr 14, 1999·Cell Death and Differentiation·F GillardonT M Michaelidis
Aug 6, 1999·Oncogene·W JiangM L Kripke
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·G S Salvesen, V M Dixit
Dec 23, 1999·The Journal of Biological Chemistry·J Z QinB J Nickoloff
Jun 30, 2000·Methods : a Companion to Methods in Enzymology·H M Shapiro
Aug 10, 2000·Nature Cell Biology·A SalehE S Alnemri
Sep 21, 2000·The Journal of Investigative Dermatology·S CichonM M Nothen
Dec 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·M RenatusG S Salvesen

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Citations

Jan 27, 2007·Archives of Dermatological Research·Catherine A KraftRichard L Eckert
Sep 10, 2005·Apoptosis : an International Journal on Programmed Cell Death·R TakasawaS Tanuma
Jun 9, 2006·Apoptosis : an International Journal on Programmed Cell Death·Ilse S DaehnTimothy E Rayner
Feb 18, 2009·Apoptosis : an International Journal on Programmed Cell Death·Saskia LippensWim Declercq
Mar 22, 2012·Apoptosis : an International Journal on Programmed Cell Death·Fenglian XiaoTongsheng Chen
May 11, 2013·Apoptosis : an International Journal on Programmed Cell Death·Weijie GaoTongsheng Chen
Nov 13, 2008·Cytotechnology·Nilou Arden, M J Betenbaugh
Jun 30, 2006·Journal of Biomedical Science·Hsin-Su YuChee-Yin Chai
Apr 3, 2009·The Journal of Physiological Sciences : JPS·Takeo YamaguchiSatoshi Katsuki
Jun 21, 2005·Biochimica Et Biophysica Acta·Zerihun AssefaPatrizia Agostinis
Feb 21, 2004·Journal of Photochemistry and Photobiology. B, Biology·Gautam Sethi, Ajit Sodhi
Mar 25, 2004·Trends in Biotechnology·Nilou Arden, Michael J Betenbaugh
Aug 7, 2010·Cell Death and Differentiation·A Hedrych-OziminaI Haase
Oct 26, 2005·Cell Death and Differentiation·S LippensW Declercq
Mar 4, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·An Van LaethemPatrizia Agostinis
Nov 18, 2009·The Journal of Biological Chemistry·Edward L LaGoryMitchell F Denning
Jun 7, 2003·American Journal of Respiratory Cell and Molecular Biology·Randolph H HastingsLeonard J Deftos
Dec 29, 2012·International Journal of Molecular Sciences·Sara SalucciElisabetta Falcieri
Apr 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Bruce C McKayJennifer C Spronck
Apr 25, 2007·Free Radical Research·Jin-Kyoung KimTae-Yoon Kim
Oct 30, 2009·Cancer Investigation·Sushma Madhukar BhosleKaushala Prasad Mishra
Jan 8, 2013·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Amel Chatti GazzahHassen Bacha
Jan 10, 2003·The Journal of Investigative Dermatology. Symposium Proceedings·Brian J NickoloffMitchell F Denning
Jan 28, 2012·Cancer Letters·Kris Nys, Patrizia Agostinis
Apr 24, 2009·Journal of Photochemistry and Photobiology. B, Biology·Nagarajan Rajendra PrasadSamivel Ramachandran
Aug 12, 2004·The Journal of Investigative Dermatology·Leonid A SitailoMitchell F Denning
Jan 23, 2009·The Journal of Investigative Dermatology·Zhongfa LuRalf Paus
Dec 17, 2003·The Journal of Investigative Dermatology·Naoko Kanda, Shinichi Watanabe
Apr 16, 2010·The Journal of Investigative Dermatology·Kris NysPatrizia Agostinis
Jul 16, 2009·The Journal of Investigative Dermatology·Frank de Gruijl
Nov 28, 2008·The Journal of Investigative Dermatology·Leonid A SitailoMitchell F Denning
May 27, 2006·The Journal of Investigative Dermatology·Tong LiuDouglas Grossman
Jan 19, 2006·The Journal of Investigative Dermatology·Deepak RajDouglas Grossman
Jul 30, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Imen Ayed-BoussemaWafa Hassen
Dec 5, 2006·Journal of Dermatological Science·Masako KuritaYoshiki Tokura
May 12, 2004·Journal of Cellular Physiology·Jian-Zhong QinBrian J Nickoloff
Aug 30, 2005·Annals of the New York Academy of Sciences·Esther ReefmanMarc Bijl

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