PMID: 9557631Apr 29, 1998Paper

Activation of caspases in pig kidney cells infected with wild-type and CrmA/SPI-2 mutants of cowpox and rabbitpox viruses

Journal of Virology
J MacenR W Moyer

Abstract

The cowpox virus (CPV) CrmA and the equivalent rabbitpox virus (RPV) SPI-2 proteins have anti-inflammatory and antiapoptosis activity by virtue of their ability to inhibit caspases, including the interleukin-1beta-converting enzyme (ICE; caspase-1). Infection of LLC-PK1 pig kidney cells with a CPV CrmA mutant, but not with wild-type (wt) CPV, results in the induction of many of the morphological features of apoptosis (C. A. Ray and D. J. Pickup, Virology 217:384-391, 1996). In our study, LLC-PK1 cells infected with CPV delta crmA, but not those infected with wt CPV, showed induction of poly(ADP-ribose) polymerase (PARP)- and lamin A-cleaving activities and processing of the CPP32 (caspase-3) precursor to a mature 18-kDa form. Surprisingly, infection of LLC-PK1 cells with either wt RPV (despite the presence of the SPI-2 protein) or RPV delta SPI-2 resulted in cleavage activity against PARP and lamin A and the appearance of the mature subunit of CPP32/caspase-3. The biotinylated specific peptide inhibitor Ac-Tyr-Val-Lys(biotinyl)-Asp-2,6-dimethylbenzoyloxymethylketone [AcYV(bio)KD-aomk] labeled active caspase subunits of 18, 19, and 21 kDa in extracts from LLC-PK1 cells infected with CPV delta crmA, wt RPV, or RPV delta SPI-2 but...Continue Reading

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Citations

Jan 25, 2003·Annual Review of Immunology·Bruce T SeetGrant McFadden
Jun 11, 1999·Immunology and Cell Biology·S TurnerJ Ruby
Oct 22, 2002·The Journal of General Virology·Zuzana HumlováZora Mělková
Aug 18, 2012·Advances in Virology·Sergei N Shchelkunov
Jun 3, 2000·Annual Review of Immunology·D TortorellaH L Ploegh
Apr 6, 2001·Seminars in Immunology·J L Shisler, B Moss
Dec 21, 2005·Virology·John M Taylor, Michele Barry

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