Activation of chloride currents in murine portal vein smooth muscle cells by membrane depolarization involves intracellular calcium release

American Journal of Physiology. Cell Physiology
Sohag N Saleh, Iain A Greenwood

Abstract

The present study describes the first characterization of Ca(2+)-activated Cl(-) currents (I(ClCa)) in single smooth muscle cells from a murine vascular preparation (portal veins). I(ClCa) was recorded using the perforated patch version of the whole cell voltage-clamp technique and was evoked using membrane depolarization. Generation of I(ClCa) relied on Ca(2+) entry through dihydropyridine-sensitive Ca(2+) channels because I(ClCa) was abolished by 1 microM nicardipine and enhanced by raising external Ca(2+) concentration or by application of BAY K 8644. I(ClCa) was characterized by the sensitivity to Cl(-) channel blockers and the effect of altering the external anion on reversal potential. Activation of I(ClCa) after membrane depolarization was dependent on Ca(2+) release from intracellular stores. Thus the amplitude of I(ClCa) was diminished by the SR-ATPase inhibitor cyclopiazonic acid, the inositol 1,4,5-trisphosphate receptor antagonist 2-aminoethoxydiphenyl borate (2-APB), and the ryanodine receptor blocker tetracaine. The degree of inhibition produced by the application of 2-APB and tetracaine together was significantly greater than the effect of each agent applied alone. In current-clamp mode, injection of depolarizing...Continue Reading

References

Aug 1, 1990·The Journal of Physiology·J J MatsudaE F Shibata
Apr 1, 1989·Pflügers Archiv : European journal of physiology·P PacaudJ Mironneau
Jul 1, 1994·Pflügers Archiv : European journal of physiology·L J Janssen, S M Sims
Jul 1, 1995·The American Journal of Physiology·L J Janssen, S M Sims
Jan 1, 1993·The Journal of Physiology·H I Akbarali, W R Giles
Oct 1, 1994·Circulation Research·F S LambE F Shibata
Feb 1, 1994·Pflügers Archiv : European journal of physiology·B NiliusH Kuriyama
Oct 1, 1996·Pflügers Archiv : European journal of physiology·I A Greenwood, W A Large
Jun 1, 1996·British Journal of Pharmacology·D N CriddleW A Large
Dec 12, 1996·European Journal of Pharmacology·A J KirkupA H Weston
Apr 15, 1997·The Journal of Physiology·S GyörkeI Györke
May 1, 1997·The American Journal of Physiology·X J Yuan
Aug 5, 1998·The American Journal of Physiology·F S Lamb, T J Barna
Jul 17, 1999·The American Journal of Physiology·F X BoittinJ Mironneau
Oct 13, 2000·American Journal of Physiology. Cell Physiology·H M TolandM A Hollywood
Apr 5, 2001·American Journal of Physiology. Cell Physiology·G P SergeantK D Thornbury
May 11, 2002·Archives of Histology and Cytology·Sumio TakahashiTatsuo Ushiki
Oct 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Jamie W MeyerGary E Shull
Mar 6, 2003·Cell Calcium·O V PovstyanT B Bolton
Mar 22, 2003·Autonomic Neuroscience : Basic & Clinical·Nick J Spencer, Iain A Greenwood
Apr 4, 2003·American Journal of Physiology. Cell Physiology·M A HollywoodK D Thornbury
Aug 26, 2003·The Journal of Physiology·Jonathan LedouxNormand Leblanc
Jul 13, 2004·The Journal of Biological Chemistry·Iain A GreenwoodNormand Leblanc

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Citations

Nov 30, 2005·Cellular and Molecular Life Sciences : CMLS·M SuzukiT Iwamoto
Jun 10, 2008·Pflügers Archiv : European journal of physiology·D M Briggs BoedtkjerC Aalkjaer
Aug 2, 2005·British Journal of Pharmacology·Shuk Yin M Yeung, Iain A Greenwood
Aug 11, 2005·Canadian Journal of Physiology and Pharmacology·Normand LeblancIain A Greenwood
Jan 24, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Simon HirotaLuke J Janssen
Apr 3, 2014·Physiological Reviews·Nicoletta Pedemonte, Luis J V Galietta
Mar 20, 2014·Channels·Eamonn BradleyGerard P Sergeant
Nov 23, 2012·Journal of Vascular Research·Vladimir V MatchkovChristian Aalkjær
Jun 26, 2018·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Shunsuke HyugaGeorge Gallos
Feb 7, 2013·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Gregory C Amberg, Manuel F Navedo
Feb 24, 2021·The Journal of Physiological Sciences : JPS·Shunsuke HyugaGeorge Gallos
Feb 9, 2021·American Journal of Physiology. Renal Physiology·Bernard T DrummGerard P Sergeant

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