Activation of deoxycytidine kinase by gamma-irradiation and inactivation by hyperosmotic shock in human lymphocytes

Biochemical Pharmacology
Zsolt CsapoMaria Sasvari-Szekely

Abstract

Deoxycytidine kinase (dCK) is a key enzyme in the intracellular metabolism of deoxynucleosides and their analogues, phosphorylating a wide range of drugs used in the chemotherapy of leukaemia and solid tumours. Previously, we found that activity of dCK can be enhanced by incubating primary cultures of lymphocytes with substrate analogues of the enzyme, as well as with various genotoxic agents. Here we present evidence that exposure of human lymphocytes to 0.5-2 Gy dosage of gamma-radiation as well as incubation of cells with calyculin A, a potent inhibitor of protein phosphatase 1 and 2A, both elevate dCK activity without changing the level of dCK protein. When cells were gamma-irradiated in the presence of calyculin A, a more pronounced activation of dCK was observed. In contrast, both basal and stimulated dCK activities were reduced by hyperosmotic treatment of the cells. DNA repair determined by the Comet assay and by thymidine incorporation was induced by irradiation. Complete repair of gamma-irradiated DNA was detected within 1 hr following the irradiation along with dCK activation, but the rate of repair was not accelerated by calyculin A. These data provide evidence for the activation of dCK upon DNA damage and repair th...Continue Reading

References

Jun 1, 1985·Molecular and Cellular Biology·P StuartS E Conrad
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M SuganumaT Sugimura
Jan 1, 1988·Acta Oto-laryngologica. Supplementum·M StaubF Antoni
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Sep 15, 1988·Biochemical and Biophysical Research Communications·T SpasokukotskajaM Staub
Jun 2, 1983·Biochimica Et Biophysica Acta·M Sasvári-SzékelyF Antoni
Jan 1, 1995·Pharmacology & Therapeutics·E S Arnér, S Eriksson
Sep 30, 1994·International Journal of Radiation Oncology, Biology, Physics·D A BoothmanW M Sahijdak
Apr 15, 1994·Pharmacy World & Science : PWS·V W Ruiz van Haperen, G J Peters
Nov 10, 1994·Biochimica Et Biophysica Acta·L M Wang, G L Kucera
Jan 15, 1993·Biochemical and Biophysical Research Communications·Q Song, M F Lavin
Oct 1, 1996·Biological & Pharmaceutical Bulletin·K OoiM Sakurai
Nov 7, 1998·The Journal of Biological Chemistry·P HatzisI Talianidis
Aug 24, 1999·The Journal of Biological Chemistry·L A Parrott, D J Templeton
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·A WoetmannN Odum
Feb 16, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T SpasokoukotskajaM Staub
Jan 10, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sara C Kozma, George Thomas
Jan 11, 2002·Advances in Experimental Medicine and Biology·T SpasokoukotskajaM Staub

❮ Previous
Next ❯

Citations

Dec 1, 2007·Chemical Communications : Chem Comm·Werner TjarksRolf F Barth
Oct 27, 2006·Nucleosides, Nucleotides & Nucleic Acids·G KeszlerM Staub
Oct 27, 2006·Nucleosides, Nucleotides & Nucleic Acids·J SigmondG J Peters
Sep 27, 2013·Radiation Oncology·Michael W LeeBo Xu
Feb 26, 2014·The Journal of Experimental Medicine·David A NathansonCaius G Radu
Feb 9, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tingting WengMichael R Blackburn
Jan 28, 2014·FEBS Letters·Rachid AmsailaleFrançoise Bontemps
Dec 2, 2015·Biochemical Pharmacology·Maxime BeyaertFrançoise Bontemps
Sep 15, 2005·Biochemical and Biophysical Research Communications·Yaacov OriAsher Korzets
Jun 9, 2004·Biochemical Pharmacology·Caroline SmalFrançoise Bontemps
Dec 2, 2004·Nucleosides, Nucleotides & Nucleic Acids·Gergely KeszlerMaria Sasvari-Szekely
Dec 2, 2004·Nucleosides, Nucleotides & Nucleic Acids·G KeszlerM Staub
Nov 24, 2016·International Journal of Molecular Sciences·Rui ZhongXiaodong Liu
Mar 9, 2005·Archives of Biochemistry and Biophysics·Gergely KeszlerMaria Staub

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

© 2022 Meta ULC. All rights reserved