PMID: 11907193Mar 22, 2002Paper

Activation of endogenous nitric oxide synthase coupled with methacholine-induced exocytosis in rat parotid acinar cells

The Journal of Pharmacology and Experimental Therapeutics
Yasuko IshikawaHajime Ishida

Abstract

Methacholine (MCh) interacted with M(3) muscarinic receptors in rat parotid tissue slices and induced amylase secretion. MCh- and calcimycin-induced exocytosis was completely inhibited by N-[2-(N-(4-chlorocinnamyl)-N-methylaminomethyl)phenyl]-N-[2-hydroxyethyl]-4-methoxybenzenesulfonamide, N(G)-nitro-L-arginine methylester (L-NAME), 1H-(1,2,4)-oxadiazolo[4,3-a]quinoxaline-1-one, and 2-(4-carboxyphenyl)-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide, suggesting that activations of calmodulin (CaM) kinase II, nitric oxide synthase (NOS), and cGMP-dependent protein kinase (PKG) were coupled with the exocytosis. These suggestions were supported by the results that exposure of the slices to MCh induced a rapid increase in these enzyme activities. Western blot analysis showed that neuronal NOS (nNOS) was expressed in isolated parotid acinar cells of rats. To measure nitric oxide (NO) production in response to the stimulation with MCh in real time, the isolated parotid acinar cells had been preloaded with 4,5-diaminofluorescein diacetate and incubated with the agonist. MCh (1 microM) induced a fast increase in 4,5-diaminofluorescein fluorescence, corresponding to an increase in the NO synthesis in the presence of extracellular Ca(2+) ...Continue Reading

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Citations

Oct 29, 2005·European Journal of Pharmacology·Adrienn BartaBrendan J R Whittle
Jun 27, 2006·Journal of Pharmacological Sciences·Yasuko IshikawaHajime Ishida
Apr 10, 2003·Pflügers Archiv : European journal of physiology·Shoji TsunodaHiroshi Sugiya
Jan 21, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Adel H SaadYoshinori Marunaka
Aug 1, 2015·American Journal of Physiology. Cell Physiology·Amira Moustafa, Yoshiaki Habara

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