Activation of endothelial cells by extracellular vesicles derived from Mycobacterium tuberculosis infected macrophages or mice

PloS One
Li LiJeffrey Schorey

Abstract

Endothelial cells play an essential role in regulating an immune response through promoting leukocyte adhesion and cell migration and production of cytokines such as TNFα. Regulation of endothelial cell immune function is tightly regulated and recent studies suggest that extracellular vesicles (EVs) are prominently involved in this process. However, the importance of EVs in regulating endothelial activation in the context of a bacterial infection is poorly understood. To begin addressing this knowledge gap we characterized the endothelial cell response to EVs released from Mycobacterium tuberculosis (Mtb) infected macrophages. Our result showed increased macrophage migration through the monolayer when endothelial cells were pretreated with EVs isolated from Mtb-infected macrophages. Transcriptome analysis showed a significant upregulation of genes involved in cell adhesion and the inflammatory process in endothelial cells treated with EVs. These results were validated by quantitative PCR and flow cytometry. Pathway analysis of these differentially expressed genes indicated that several immune response-related pathways were up-regulated. Endothelial cells were also treated with EVs isolated from the serum of Mtb-infected mice. I...Continue Reading

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Citations

May 13, 2020·Scandinavian Journal of Gastroenterology·Marlene Christina NielsenHolger Jon Møller
Sep 3, 2019·Frontiers in Immunology·Jianjun WangRodolfo C Garcia
May 14, 2020·Cells·Marlene Christina NielsenHenning Grønbæk
Apr 17, 2021·Biochemical Society Transactions·Joni Renee WhiteSimon Swift

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Methods Mentioned

BETA
FACS
confocal microscopy
RNAseq
RNA-seq
Flow cytometry
nuclear translocation
PCR

Software Mentioned

Tophat
NTA
METACORE
Trimmomatic
cuffdiff
bowtie2
FastQC
CXP Analysis
miSeq

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