Activation of endothelial nitric oxide synthase following spinal cord injury in mice

Neuroscience Letters
Koji OsukaMasakazu Takayasu

Abstract

Endothelial nitric oxide synthase (eNOS) plays a neuroprotective role after cerebral ischemia through the production of NO, which enhances cerebral blood flow. However, precise details regarding activation of eNOS after spinal cord injury (SCI) largely remain to be elucidated. In the present study we investigated chronological alteration and cellular location of eNOS and phosphorylated (p)-eNOS at Ser(1177) following SCI in mice. Western blot analysis showed eNOS to be significantly phosphorylated at Ser(1177) from 1 to 2 days after mild SCI, with gradual decrease thereafter. Immunohistochemistry revealed the p-eNOS to be mainly expressed in the endothelial cells of microvessels within gray matter under these conditions. These findings suggest that mild SCI activates eNOS in the subacute stage, which increases spinal cord blood flow and may be involved in protective and repair responses.

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Citations

Aug 10, 2010·The Journal of Trauma·Abdieel Esquivel-AguilarGabriel Guízar-Sahagún
Apr 5, 2014·The International Journal of Neuroscience·Valentina TardivoDiego Garbossa
Oct 14, 2014·Frontiers in Cellular Neuroscience·Mayada A MahmoudIsmail Sabry
Mar 19, 2009·Pharmacological Reviews·Noboru TodaTomio Okamura

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