Activation of ephrin A proteins influences hematopoietic stem cell adhesion and trafficking patterns

Experimental Hematology
Michael J TingAndrew W Boyd

Abstract

To determine if Eph receptors and ephrins can modulate the homing of hematopoietic cells in a murine bone marrow transplantation model. EphA and ephrin A gene expression by mouse hematopoietic stem cells and the progenitor cell line FDCP-1 was determined by real-time reverse transcription polymerase chain reaction and flow cytometry. The effect of ephrin A activation on adhesion of hematopoietic progenitors was determined by in vitro adhesion assays in which cells were exposed to fibronectin or vascular cell adhesion molecule-1 (VCAM-1) and an increasing gradient of immobilized EphA3-Fc. Adhesion to fibronectin and VCAM-1 was further investigated using soluble preclustered EphA3-Fc. We used soluble unclustered EphA3-Fc as an antagonist to block endogenous EphA-ephrin A interactions in vivo. The effect of injecting soluble EphA3-Fc on the mobilization of hematopoietic progenitor cells was examined. We determined the effect on short-term homing by pretreating bone marrow cells with EphA3-Fc or the control IgG before infusion into lethally irradiated mice. Preclustered and immobilized EphA3-Fc increased adhesion of progenitor cells and FDCP-1 to fibronectin and VCAM-1 (1.6- to 2-fold higher adhesion; p < 0.05) relative to control ...Continue Reading

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Citations

Mar 23, 2011·Stem Cell Research & Therapy·Jonathan Hoggatt, Louis M Pelus
Nov 11, 2011·Development·Danny A StarkD D W Cornelison
Oct 18, 2015·International Journal of Hematology·Thao M NguyenStan Gronthos
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Aug 6, 2020·Frontiers in Cell and Developmental Biology·Rohan Kulkarni, Vaijayanti Kale
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Feb 27, 2021·Frontiers in Cell and Developmental Biology·Agnieszka Arthur, Stan Gronthos

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