Activation of epidermal growth factor receptor via CCR3 in bronchial epithelial cells

Biochemical and Biophysical Research Communications
Tetsuya AdachiJunichi Chihara

Abstract

We have previously found that bronchial epithelial cells express CCR3 whose signaling elicits mitogen-activated protein (MAP) kinase activation and cytokine production. Several investigators have focused on the signaling crosstalk between G protein-coupled receptors (GPCRs) and epidermal growth factor receptor (EGFR) in cancer cells. In this study, we investigated the role of EGFR in CCR3 signaling in the bronchial epithelial cell line NCI-H292. Eotaxin (1-100 nM) induced dose-dependent tyrosine phosphorylation of EGFR in NCI-H292 cells. Pretreatment of the cells with the EGFR inhibitor (AG1478) significantly inhibited the MAP kinase phosphorylation induced by eotaxin. Eotaxin stimulated IL-8 production, which was inhibited by AG1478. The transactivation of EGFR through CCR3 is a critical pathway that elicits MAP kinase activation and cytokine production in bronchial epithelial cells. The delineation of the signaling pathway of chemokines will help to develop a new therapeutic strategy to allergic diseases including bronchial asthma.

References

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Jul 2, 2002·American Journal of Respiratory Cell and Molecular Biology·Audrey RichterDonna E Davies
Sep 3, 2002·American Journal of Respiratory Cell and Molecular Biology·Chang-Hao CuiJunichi Chihara
Jan 11, 2003·The Journal of Biological Chemistry·Abdelkarim SabriSusan F Steinberg

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Citations

Dec 23, 2008·Cellular and Molecular Life Sciences : CMLS·C L SalangaT Handel
Oct 9, 2008·Bulletin of Mathematical Biology·E A GaffneyM Z Kwiatkowska
Jun 15, 2006·International Archives of Allergy and Immunology·Tetsuya AdachiKen Ohta
Jun 11, 2011·Journal of Cellular Physiology·Bo LiChunxue Bai
Apr 9, 2008·Journal of Theoretical Biology·Sohyun HwangDoheon Lee
Oct 26, 2006·Journal of Cellular Biochemistry·Hao-Teng ChangMargaret Dah-Tsyr Chang
May 3, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·B O AbonyoA S Heiman
Jul 1, 2005·Expert Review of Clinical Immunology·Magdalena M Gorska, Rafeul Alam
Sep 21, 2005·The Journal of Biological Chemistry·Chareeporn AkekawatchaiShaun R McColl

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