PMID: 16618982Apr 19, 2006Paper

Activation of follistatin promoter by GnRH in LbetaT2 gonadotroph cells

Endocrine Journal
Sachiko KiharaHitoshi Okamura

Abstract

Follistatin (FS) is produced and secreted from gonadotroph cells in pituitary gland as well as granulosa cells in the ovary. In the present study, we found that the FS promoter is activated by GnRH in the gonadotroph cell line, LbetaT2. Therefore, we examined the signal transduction pathways involved in the mechanism. The activation of the FS promoter by GnRH was inhibited by calphostin C, a protein kinase C inhibitor, and U0126, a MAP kinase kinase (MEK) inhibitor. Phosphorylation by protein kinase C of myristoylated alanine-rich C kinase substrate (MARCKS) in LbetaT2 cells was observed after 3-min treatment with GnRH and declined after 30 min. The subsequent activation of MAP kinase was also transient, and down-regulation of protein kinase C completely inhibited the MAP kinase activation by GnRH, suggesting that the transient activation of protein kinase C led to the transient activation of MAP kinase. Although phorbol 12-myristate 13-acetate treatment increased phosphorylation of MARCKS and activated MAP kinase, it did not activate the FS promoter. Genistein, a tyrosine kinase inhibitor, completely inhibited the GnRH-induced activation of the FS promoter, while no inhibition of the MAP kinase pathway was observed. These resu...Continue Reading

References

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Citations

Mar 24, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Nataša NestorovićVerica Milošević
Feb 15, 2012·Molecular and Cellular Endocrinology·Louise M BilezikjianWylie W Vale

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