Activation of H+-ATPase of the plasma membrane of Saccharomyces cerevisiae by glucose: the role of sphingolipid and lateral enzyme mobility.

PloS One
Sergey PermyakovAirat Valiakhmetov

Abstract

Activation of the plasma membrane H(+)-ATPase of the yeast Saccharomyces cerevisiae by glucose is a complex process that has not yet been completely elucidated. This study aimed to shed light on the role of lipids and the lateral mobility of the enzyme complex during its activation by glucose. The significance of H(+)-ATPase oligomerization for the activation of H(+)-ATPase by glucose was shown using the strains lcb1-100 and erg6, with the disturbed synthesis of sphyngolipid and ergosterol, respectively. Experiments with GFP-fused H(+)-ATPase showed a decrease in fluorescence anisotropy during the course of glucose activation, suggesting structural reorganization of the molecular domains. An immunogold assay showed that the incubation with glucose results in the spatial redistribution of ATPase complexes in the plasma membrane. The data suggest that (1) to be activated by glucose, H(+)-ATPase is supposed to be in an oligomeric state, and (2) glucose activation is accompanied by the spatial movements of H(+)-ATPase clusters in the PM.

References

Nov 30, 1992·Annals of the New York Academy of Sciences·R RaoC W Slayman
Sep 25, 1992·Biochimica Et Biophysica Acta·D Seto-YoungD S Perlin
Feb 1, 1987·Archives of Biochemistry and Biophysics·C C ChadwickG A Scarborough
Dec 1, 1974·Analytical Biochemistry·D J Stewart
Dec 5, 1995·Biochemistry·S Lutsenko, J H Kaplan
Oct 1, 1995·Biophysical Journal·L W Runnels, S F Scarlata
Mar 21, 1998·The Journal of Cell Biology·A PartikianA S Verkman
Feb 26, 1999·Analytical Biochemistry·J R LakowiczJ D Dattelbaum
Mar 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·M BagnatK Simons
Apr 5, 2000·The Journal of Biological Chemistry·M A HinkA J Visser
Dec 12, 2001·Molecular Biology of the Cell·M BagnatK Simons
Sep 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Qiongqing Wang, Amy Chang
Oct 11, 2003·Molecular Biology of the Cell·Katerina MalínskáWidmar Tanner
Dec 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Björn F LillemeierMark M Davis
Nov 4, 2009·Biophysical Journal·Arjen N BaderHans C Gerritsen
Feb 24, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Arjen N BaderHans C Gerritsen
Mar 1, 2011·Annual Review of Biophysics·Michael G Palmgren, Poul Nissen

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Citations

Apr 8, 2014·Biophysical Journal·Zahra Zolmajd-Haghighi, Quentin S Hanley
Feb 21, 2019·FEMS Yeast Research·A Ya ValiakhmetovA O Shepelyakovskaya
May 11, 2018·FEBS Open Bio·Jesper Torbøl PedersenMichael Palmgren
Mar 13, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yadira G Ruiz-GranadosJosé G Sampedro

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