Activation of heat shock gene transcription by heat shock factor 1 involves oligomerization, acquisition of DNA-binding activity, and nuclear localization and can occur in the absence of stress.

Molecular and Cellular Biology
Kevin D SargeR I Morimoto

Abstract

The existence of multiple heat shock factor (HSF) genes in higher eukaryotes has promoted questions regarding the functions of these HSF family members, especially with respect to the stress response. To address these questions, we have used polyclonal antisera raised against mouse HSF1 and HSF2 to examine the biochemical, physical, and functional properties of these two factors in unstressed and heat-shocked mouse and human cells. We have identified HSF1 as the mediator of stress-induced heat shock gene transcription. HSF1 displays stress-induced DNA-binding activity, oligomerization, and nuclear localization, while HSF2 does not. Also, HSF1 undergoes phosphorylation in cells exposed to heat or cadmium sulfate but not in cells treated with the amino acid analog L-azetidine-2-carboxylic acid, indicating that phosphorylation of HSF1 is not essential for its activation. Interestingly, HSF1 and HSF2 overexpressed in transfected 3T3 cells both display constitutive DNA-binding activity, oligomerization, and transcriptional activity. These results demonstrate that HSF1 can be activated in the absence of physiological stress and also provide support for a model of regulation of HSF1 and HSF2 activity by a titratable negative regulator...Continue Reading

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K LiberekC Georgopoulos
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S K RabindranC Wu
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T J SchuetzR E Kingston
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D D MosserR I Morimoto
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P GunningL Kedes
Mar 1, 1989·Journal of Bacteriology·K TillyC Georgopoulos
Jan 1, 1983·Biology of the Cell·D L SpectorH Busch
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard

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Citations

Sep 3, 2005·Immunological Investigations·Ashish NagarsekarIshwar S Singh
Sep 1, 1995·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·H Ovelgönne, R Van Wijk
May 1, 1994·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·N F MivechiG M Hahn
Feb 1, 1997·Annals of Medicine·S Leppä, L Sistonen
Jan 5, 2016·Nature Structural & Molecular Biology·Tobias NeudeggerAndreas Bracher
Jun 17, 1994·Annals of the New York Academy of Sciences·A A Knowlton
Mar 31, 2009·Journal of Theoretical Biology·Zuzanna Szymańska, Maciej Zylicz
Apr 11, 2013·Autoimmune Diseases·E W BrenuS M Marshall-Gradisnik
Oct 15, 2010·The FEBS Journal·Mitsuaki Fujimoto, Akira Nakai
Jul 29, 2005·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·J Tonkiss, S K Calderwood
Aug 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anniina VihervaaraLea Sistonen
Nov 19, 2014·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Priyanka PatelJasna Kriz
Oct 22, 2009·Human Molecular Genetics·Christopher P ReinaRandall N Pittman
Aug 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Malin AkerfeltLea Sistonen
Feb 21, 2009·Science·Sandy D WesterheideRichard I Morimoto
Mar 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Frank BoellmannRichard Voellmy
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·C JollyR I Morimoto
Nov 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kaoru SakabeGerald W Hart
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Eniko NagyLászló Vígh
Sep 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Andrew D SteeleSusan Lindquist
Nov 20, 2016·Cold Spring Harbor Perspectives in Biology·Tatyana DubnikovEhud Cohen
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·M RalluV Mezger
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·K KaarnirantaH J Helminen
Jul 10, 1998·The Proceedings of the Nutrition Society·M J JacksonF McArdle
Mar 30, 1999·Biochimica Et Biophysica Acta·C R HuntC A Kozak
Jan 5, 2017·The FEBS Journal·Sharadha Dayalan Naidu, Albena T Dinkova-Kostova
Jan 7, 2000·Journal of Molecular Biology·J A HardyH C Nelson

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