PMID: 9192693Jun 24, 1997Paper

Activation of heteromeric G protein-gated inward rectifier K+ channels overexpressed by adenovirus gene transfer inhibits the excitability of hippocampal neurons

Proceedings of the National Academy of Sciences of the United States of America
M U EhrengruberN Davidson

Abstract

G protein-gated inward rectifier K+ channel subunits 1-4 (GIRK1-4) have been cloned from neuronal and atrial tissue and function as heterotetramers. To examine the inhibition of neuronal excitation by GIRKs, we overexpressed GIRKs in cultured hippocampal neurons from 18 day rat embryos, which normally lack or show low amounts of GIRK protein and currents. Adenoviral recombinants containing the cDNAs for GIRK1, GIRK2, GIRK4, and the serotonin 1A receptor were constructed. Typical GIRK currents could be activated by endogenous GABAB, serotonin 5-HT1A, and adenosine A1 receptors in neurons coinfected with GIRK1+2 or GIRK1+4. Under current clamp, GIRK activation increased the cell membrane conductance by 1- to 2-fold, hyperpolarized the cell by 11-14 mV, and inhibited action potential firing by increasing the threshold current for firing by 2- to 3-fold. These effects were not found in non- and mock-infected neurons, and were similar to the effects of muscarinic stimulation of native GIRK currents in atrial myocytes. Two inhibitory effects of GIRK activation, hyperpolarization and diminution of depolarizing pulses, were simulated from the experimental data. These inhibitory effects are physiologically important in the voltage range...Continue Reading

References

Apr 1, 1991·The Journal of Membrane Biology·P H Barry, J W Lynch
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A KarschinH A Lester
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L E IversonB Rudy
Sep 1, 1989·British Journal of Pharmacology·R A North
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S EfratS Baekkeskov
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B H Gähwiler, D A Brown
Apr 1, 1988·The Journal of Physiology·D V Madison, R A Nicoll
Jun 16, 1995·The Journal of Biological Chemistry·L H PhilipsonR J Miller
Jun 1, 1995·Current Opinion in Neurobiology·C A DoupnikH A Lester
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·P KofujiH A Lester
Aug 1, 1995·Journal of Neurochemistry·L D MorrisonS J Kish
Aug 11, 1994·Nature·M L AshfordJ P Adelman
May 1, 1994·Journal of Neurocytology·D L BensonG Banker
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·N DascalD Trollinger
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·S SignoriniM Stoffel
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·C T DrakeC Chavkin

❮ Previous
Next ❯

Citations

Mar 30, 1988·Biochimica Et Biophysica Acta·M YohdaY Kagawa
Mar 20, 1993·Biochimica Et Biophysica Acta·D L XieN Nelson
Jul 22, 1998·European Journal of Pharmacology·Y EdagawaK Abe
Aug 11, 2000·Journal of Neuroscience Methods·H Nadeau, H A Lester
Oct 10, 2002·Brain Research Bulletin·Markus U Ehrengruber
Mar 5, 2013·Nature Neuroscience·Joshua LevitzEhud Y Isacoff
Aug 10, 2002·The European Journal of Neuroscience·Ulf BickmeyerDiethelm W Richter
Jan 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hee Jung ChungLily Yeh Jan
Jan 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hee Jung ChungLily Yeh Jan
Feb 3, 1999·Annals of the New York Academy of Sciences·R Andrade
Jul 22, 1999·Annals of the New York Academy of Sciences·D C JohnsH B Nuss
Jun 9, 2000·Annual Review of Neuroscience·H A Lester, A Karschin
May 31, 2011·Molecular Brain·Paul R AlbertAnne M Millar
Jul 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·P LorenteG Vassort
Nov 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xiang LiStefan Herlitze
Jul 9, 2014·Annual Review of Neuroscience·Scott M Sternson, Bryan L Roth
Aug 12, 2015·International Journal of Molecular Sciences·Zdzisław ChilmonczykIngebrigt Sylte
Jan 22, 2013·Trends in Neurosciences·Raquel B DiasAna M Sebastião
Jul 2, 2011·Pharmacology & Therapeutics·Jean-Luc GaiarsaChristophe Porcher
Oct 4, 2008·The American Journal of Pathology·Muriel B SättlerRicarda Diem
Oct 19, 2006·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Katheleen Gardiner, Alberto C S Costa
Nov 18, 2005·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Llorenç Díaz-MataixPau Celada
Jul 5, 2005·Neuropharmacology·Richard J SiareyZygmunt Galdzicki
Aug 2, 2003·Biochemical and Biophysical Research Communications·Byron D FordGerald D Fischbach
Nov 29, 2011·Current Opinion in Neurobiology·Peer Wulff, Benjamin R Arenkiel
Apr 6, 2007·Neuron·Susan M Dymecki, Jun Chul Kim

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atrial Fibrillation

Atrial fibrillation is a common arrhythmia that is associated with substantial morbidity and mortality, particularly due to stroke and thromboembolism. Here is the latest research.

Atrial Filbrillation

Atrial fibrillation refers to the abnormal heart rhythm characterized by rapid and irregular beating of the atria. Here is the latest research.

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.