Activation of homologous recombination DNA repair in human skin fibroblasts continuously exposed to X-ray radiation

Oncotarget
Andreyan N OsipovIlya Eremin

Abstract

Molecular and cellular responses to protracted ionizing radiation exposures are poorly understood. Using immunofluorescence microscopy, we studied the kinetics of DNA repair foci formation in normal human fibroblasts exposed to X-rays at a dose rate of 4.5 mGy/min for up to 6 h. We showed that both the number of γH2AX foci and their integral fluorescence intensity grew linearly with time of irradiation up to 2 h. A plateau was observed between 2 and 6 h of exposure, indicating a state of balance between formation and repair of DNA double-strand breaks. In contrast, the number and intensity of foci formed by homologous recombination protein RAD51 demonstrated a continuous increase during 6 h of irradiation. We further showed that the enhancement of the homologous recombination repair was not due to redistribution of cell cycle phases. Our results indicate that continuous irradiation of normal human cells triggers DNA repair responses that are different from those elicited after acute irradiation. The observed activation of the error-free homologous recombination DNA double-strand break repair pathway suggests compensatory adaptive mechanisms that may help alleviate long-term biological consequences and could potentially be utili...Continue Reading

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Citations

Feb 6, 2017·The Plant Journal : for Cell and Molecular Biology·Takeshi HirakawaSachihiro Matsunaga
May 31, 2019·International Journal of Molecular Sciences·Stepan UlyanenkoAndreyan N Osipov
Sep 17, 2020·International Journal of Molecular Sciences·Usha KabilanDmitry Klokov
Dec 14, 2016·Aging·Margarita PustovalovaAndreyan N Osipov
Feb 18, 2016·Oncotarget·Claire ViallardFrançoise Degoul
May 2, 2019·Dose-response : a Publication of International Hormesis Society·Zhen WangYuta Shibamoto

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Methods Mentioned

BETA
X-ray
biopsies
biopsy

Software Mentioned

Statistica

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