Activation of human dendritic cells by bacillus Calmette-Guerin

The Journal of Urology
R RamonerM Thurnher

Abstract

Dendritic cells are the most potent antigen presenting cells capable of initiating antitumor immune responses. We previously showed that bacillus Calmette-Guerin (BCG) stimulates cultured human dendritic cells. We extended these studies and tested the ability of cultured human dendritic cells to express interleukin IL-8 in response to BCG. We also investigated the T cell stimulatory potential of BCG treated dendritic cells in mixed leukocyte reactions. Dendritic cells were obtained by culturing plastic adherent mononuclear cells from peripheral blood for 6 days in the presence of granulocyte-macrophage colony-stimulating factor and IL-4. Spontaneous and BCG stimulated IL-8 protein release into culture supernatants was measured by a quantitative immunoassay. IL-8 gene transcription was assessed by reverse transcription-polymerase chain reaction. Untreated and BCG exposed dendritic cells were compared as stimulators of allogeneic T cell proliferation, measured as [3H]thymidine incorporation. BCG stimulated IL-8 messenger ribonucleic acid expression and IL-8 protein release. IL-8 secretion occurred in a dose and time dependent fashion. BCG induced IL-8 release was further enhanced in the presence of indomethacin. BCG treated dendr...Continue Reading

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Citations

Aug 18, 2000·Immunology and Cell Biology·B M Saunders, A M Cooper
Feb 27, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·C JagannathR L Hunter
Jul 20, 2010·Immunotherapy·Anton B AlexandroffAndrew M Jackson
Dec 21, 2000·Clinical and Experimental Immunology·M SchoppetH I Huppertz
Feb 29, 2008·Immunopharmacology and Immunotoxicology·Kambiz BagheriSeyed-Mohammad Moazzeni
Sep 18, 2004·European Journal of Immunology·Francesco DieliAlfredo Salerno
Sep 15, 2006·Journal of Leukocyte Biology·Martin Thurnher
Jul 26, 2018·Archives of Virology·Jean-Marie Andrieu, Wei Lu
Apr 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ingo FrickeSven Brandau
Mar 7, 2021·Journal of Clinical Medicine·Renate PichlerMartin Thurnher

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