PMID: 8588976Aug 1, 1995Paper

Activation of human endometrial phospholipase D by bradykinin

Cellular Signalling
A AhmedS K Smith

Abstract

Bradykinin may act as a promoter of endometrial regeneration. In [3H]myristate-labelled endometrial stromal cells, bradykinin and tetradecanoylphorbol acetate (TPA) mediated activation of phospholipase D (PLD) as measured by the accumulation of [3H]phosphatidylbutanol ([3H]PtdBut). Kinetics of bradykinin-evoked PLD activation was rapid and transient, whereas the TPA response was relatively slow in onset. Bradykinin induced a dose-dependent (EC50 0.11 nM) [3H]PtdBut accumulation at concentrations at which it stimulated DNA synthesis. In [3H]inositol-labelled cells, bradykinin evoked a rapid increase in inositol phosphates which preceded the increase in [3H]PtdBut formation. Chronic pretreatment with 400 nM TPA abolished PLD activation to subsequent treatment with either TPA and bradykinin. Staurosporine, an inhibitor of protein kinase C, strongly inhibited (IC50 96 nM) TPA-induced [3H]PtdBut formation, but bradykinin-stimulated [3H]PtdBut accumulation was only partially inhibited (IC50 65 microM). The effect of bradykinin and TPA on PLD activity was synergistic, suggesting that the two agents may act via different mechanisms. These results suggest PKC-dependent and independent pathways are involved in bradykinin-induced PLD acti...Continue Reading

References

Oct 1, 1992·Trends in Biochemical Sciences·R G Knowles, S Moncada
Dec 1, 1992·Baillière's Clinical Obstetrics and Gynaecology·A S Ahmed, S K Smith
Jul 1, 1992·Journal of Cellular Physiology·A Ahmed, S K Smith
Nov 1, 1991·Trends in Pharmacological Sciences·N T ThompsonL G Garland
Aug 1, 1991·Molecular and Cellular Endocrinology·A M Vinggaard, H S Hansen
Feb 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S L ReinholdT M McIntyre
Jul 15, 1990·The Biochemical Journal·M M Billah, J C Anthes
Mar 1, 1988·Journal of Reproduction and Fertility·A C McRae, R B Heap
Mar 13, 1986·Biochemical and Biophysical Research Communications·T TamaokiF Tomita
Aug 1, 1994·The American Journal of Physiology·H MatsunagaD C Eaton
Jan 28, 1993·Nature·M J Berridge

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Citations

Mar 18, 2020·Acta Obstetricia Et Gynecologica Scandinavica·Antonio RaffoneFulvio Zullo
Mar 30, 2018·Frontiers in Endocrinology·Yannick Audet-DelageChantal Guillemette

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