PMID: 2105252Feb 1, 1990Paper

Activation of human neutrophil phospholipase D by three separable mechanisms

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
S L ReinholdT M McIntyre

Abstract

Activation of human neutrophils by receptor-mediated agonists, the Ca2+ ionophore A23187, or the protein kinase C activator phorbol myristate acetate all stimulated phospholipase D activity. This was demonstrated by the increased formation of phosphatidic acid, and in the presence of ethanol, phosphatidylethanol (PEt) accumulation. EGTA completely inhibited A23187-induced PEt formation, but only one-half of the fMLP-induced PEt accumulation. Staurosporin, an inhibitor of protein kinase C, strongly inhibited PMA-induced PEt formation, but actually stimulated the formation of PEt in response to fMLP by several-fold. Thus, increased cytosolic Ca2+ and activated protein kinase C can each lead to activation of phospholipase D, but neither is required for receptor-mediated activation of phospholipase D activity. Wortmannin is an irreversible inhibitor of the oxidative burst, but does not inhibit NADPH oxidase or known components of signal transduction. Wortmannin inhibited activation of phospholipase D in response to fMPL. It did not directly inhibit phospholipase D, as the response to A23187 was unaffected. Wortmannin did not inhibit other fMPL-stimulated events, such as aggregation or adherence. We conclude that inhibition by wortm...Continue Reading

Citations

Mar 1, 1992·The American Review of Respiratory Disease·N F VoelkelR C Murphy
May 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·C J WelshK McBride
May 28, 1993·Annals of the New York Academy of Sciences·L van AntwerpenR Anderson
Jun 5, 1998·The American Journal of Physiology·J H JaggarM T Nelson
Dec 1, 1994·Archives of Pharmacal Research·J H JungS H Chung
Aug 31, 1999·Immunopharmacology and Immunotoxicology·M A Panaro, V Mitolo
Jun 12, 2003·The Journal of Biological Chemistry·Rory MitchellMelanie S Johnson
Sep 1, 1993·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J Shute
Sep 16, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M PouliotP H Naccache
Feb 1, 1994·British Journal of Pharmacology·H JinoK Kurahashi
Jun 1, 1993·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E F KnolD Roos
Feb 1, 1997·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·C BonneauJ P Giroud
Mar 1, 1993·European Journal of Biochemistry·D De ValckW Fiers
May 1, 1991·British Journal of Pharmacology·R W BonserL G Garland

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