PMID: 7932652Jun 1, 1994Paper

Activation of hyperpolarization and atypical osmosensitivity of a Cl- current in rat osteoblastic cells

The Journal of Membrane Biology
D Chesnoy-Marchais, J Fritsch

Abstract

During whole-cell recording of rat osteoblastic cells with high-Cl- internal solutions, 10 sec hyperpolarizing jumps from 0 mV induce a slow inward current relaxation, which is shown to be carried by hyperpolarization-activated Cl- channels. This relaxation increases and becomes faster with stronger hyperpolarizations. It is insensitive to Cs+ ions but is blocked in a voltage-dependent manner by 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) 1 mM and is reduced by 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) 0.1 mM. Cd2+ ions are potent blockers of this current, blocking completely above 300 microM. The amplitude of the Cl- current activated by a given hyperpolarization increases during the first 10-20 min of whole-cell recording. This evolution and the fact that some recently cloned Cl- channels have been reported to be activated both by hyperpolarization and by external hyposmolarity led us to investigate the effects of external osmolarity. Reducing the external osmolarity induces a large Cl- current. However, this hyposmolarity-induced Cl- current and the hyperpolarization-activated Cl- current are shown to be distinct; 1,9-dideoxy forskolin selectively blocks the hyposmolarity-activated current. We show th...Continue Reading

Citations

Jan 1, 1997·Progress in Biophysics and Molecular Biology·B NiliusG Droogmans
Jun 1, 1996·Current Opinion in Neurobiology·T J Jentsch
Apr 1, 1997·The EMBO Journal·S E Jordt, T J Jentsch
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·C LorenzT J Jentsch
May 17, 2002·The Journal of Physiology·Jorge ArreolaJames E Melvin
Feb 27, 2003·The European Journal of Neuroscience·Dominique Chesnoy-Marchais
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J E Melvin
Oct 27, 2005·Journal of Cellular Physiology·Steven HughesAlicia J El Haj
Mar 28, 2002·Physiological Reviews·Thomas J JentschAnselm A Zdebik
Mar 1, 1997·The American Journal of Physiology·J Fritsch, A Edelman
May 15, 2001·American Journal of Physiology. Cell Physiology·M BaliJ Fritsch

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