Activation of in situ tissue transglutaminase by intracellular reactive oxygen species

Biochemical and Biophysical Research Communications
Zee-Won LeeKwon-Soo Ha

Abstract

We have investigated the novel function of intracellular reactive oxygen species (ROS) in the activation of in situ tissue transglutaminase (tTGase) by lysophosphatidic acid (LPA) and transforming growth factor-beta (TGF-beta) in Swiss 3T3 fibroblasts. LPA induced a transient increase of intracellular ROS with a maximal increase at 10 min, which was blocked by ROS scavengers, N-acetyl-L-cysteine and catalase. LPA activated tTGase with a maximal increase at 1h, which was inhibited by cystamine and ROS scavengers. Incubation with exogenous H(2)O(2) activated tTGase. TGF-beta also activated tTGase with a maximal activation at 2h and the tTGase activation was inhibited by the ROS scavengers. Scrape-loading of C3 transferase inhibited the ROS production and in situ tTGase activation by LPA and TGF-beta, and the inhibitory effect of C3 transferase was reversed by exogenous H(2)O(2). Microinjection of GTPgammaS inhibited transamidating activity of tTGase stimulated by LPA, TGF-beta, and maitotoxin. These results suggested that intracellular ROS was essential for the activation of in situ tTGase in response to LPA and TGF-beta.

References

Jun 1, 1995·Trends in Biochemical Sciences·Y TakaiH Nakanishi
Aug 2, 1996·The Journal of Biological Chemistry·E BallestarL Franco
Jun 27, 1997·The Journal of Biological Chemistry·T S LaiC S Greenberg
Jan 27, 1998·The Journal of Biological Chemistry·J ZhangG V Johnson
Jun 20, 1998·The Journal of Biological Chemistry·M LesortG V Johnson
Jul 20, 1999·Experimental & Molecular Medicine·S G Rhee
Sep 11, 1999·The International Journal of Biochemistry & Cell Biology·J S Chen, K Mehta
Nov 7, 1999·The Journal of Biological Chemistry·G U BaeJ W Han
Feb 8, 2000·The Journal of Biological Chemistry·A Di VenereG Mei
Feb 23, 2000·The Journal of Cell Biology·S S AkimovA M Belkin
Aug 14, 2001·Biochemical and Biophysical Research Communications·S W KimK S Ha

❮ Previous
Next ❯

Citations

Mar 29, 2007·Amino Acids·R IentileM Griffin
Aug 2, 2011·Amino Acids·Daniela CaccamoRiccardo Ientile
Feb 4, 2009·Antioxidants & Redox Signaling·Hanke L MatlungEd VanBavel
Feb 23, 2011·Development, Growth & Differentiation·Rocío Hernández-Martínez, Luis Covarrubias
Jun 28, 2011·American Journal of Respiratory and Critical Care Medicine·Keith C OlsenPatricia J Sime
Jul 9, 2008·BMC Neuroscience·Nadia FerlazzoDaniela Caccamo
Nov 30, 2011·Drug Discovery Today. Disease Models·Luis A Martinez-Lemus, Edgar Luis Galiñanes
Apr 9, 2009·Journal of Biomedical Materials Research. Part a·D I ZeugolisM Raghunath
Nov 16, 2004·Biochemical and Biophysical Research Communications·Sun-Ju YiKwon-Soo Ha
Jul 29, 2018·Bioscience Reports·Thomas M JeitnerArthur J L Cooper
Jan 1, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·K C Penumatsa, B L Fanburg
Mar 26, 2010·Biological Trace Element Research·Esteban MezeyJames J Potter
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chinmayee D BhediKrishna C Penumatsa
Mar 15, 2020·Experimental Lung Research·Elke BoxhammerMarietta von Suesskind-Schwendi
Jul 26, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Frank SovegJoan M Cook-Mills
Jul 8, 2017·Scientific Reports·Ronak ShresthaSoichi Kojima
Mar 15, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Morten A KarsdalDetlef Schuppan
Sep 30, 2021·Biomedical Materials·Wenyu KongYanan Du
Oct 28, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kempaiah RayavaraYashoda M Hosakote
Jul 25, 2017·Advanced Drug Delivery Reviews·M A KarsdalD Schuppan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Botulism (ASM)

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Botulism

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.