Activation of inducible nitric oxide synthase by Yongdam-Sagan-Tang in mouse peritoneal macrophages

Journal of Ethnopharmacology
E H LeeH M Kim

Abstract

The objective of the current study was to determine the effect of Yongdam-Sagan-Tang (YS-Tang) on the production of nitric oxide (NO). Stimulation of mouse peritoneal macrophages with YS-Tang after the treatment of recombinant interferon-gamma (rIFN-gamma) resulted in increased NO synthesis. YS-Tang had no effect on NO synthesis by itself. When YS-Tang was used in combination with rIFN-gamma, there was a marked co-operative induction of NO synthesis in a dose-dependent manner. The optimal effect of YS-Tang on NO synthesis was shown 6 h after treatment with rIFN-gamma. This increase in NO synthesis was reflected as an increased amount of inducible NO synthase (iNOS) protein. NO production was inhibited by NG-monomethyl-L-arginine. The increased production of NO from rIFN-gamma plus YS-Tang-stimulated cells was decreased by the treatment with staurosporin. In addition, synergy between rIFN-gamma and YS-Tang was mainly dependent on YS-Tang-induced tumor necrosis factor-alpha (TNF-alpha) secretion. All the preparations of YS-Tang were endotoxin free. These results suggest that the capacity of YS-Tang to increase NO production from rIFN-gamma-primed mouse peritoneal macrophages is the result of YS-Tang-induced TNF-alpha secretion.

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Citations

Mar 7, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hwan-Suck ChungHyung-Min Kim
Jul 10, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hyun-Ja JeongHyung-Min Kim
Sep 21, 2002·Journal of Ethnopharmacology·Hwan-Suck ChungHyung-Min Kim
Jan 5, 2000·Journal of Ethnopharmacology·H M KimN H An
Jul 16, 2003·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Hwan-Suck ChungHyung-Min Kim
Sep 17, 2002·Biological & Pharmaceutical Bulletin·Hwan-Suck ChungHyung-Min Kim

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