Activation of intestinal guanylate cyclase by heat-stable enterotoxin of Escherichia coli: studies of tissue specificity, potential receptors, and intermediates

The Journal of Infectious Diseases
R L GuerrantF Murad

Abstract

Heat-stable enterotoxin (ST) of Escherichia coli increased guanylate cyclase activity in homogenates of rat and rabbit intestinal mucosa and stimulated intestinal fluid secretion in suckling mice. The ST effect on guanylate cyclase was dose-dependent, occurred without a time lag, and was confined to the particulate fraction. ST activation of guanylate cyclase was tissue-specific; ST did not alter activity of soluble or particulate rat liver, lung, heart, kidney, or cerebral cortex enzyme. The ST activity on guanylate cyclase and secretion was methanol-soluble and alkali-labile, and its effects were not altered by phentolamine, propranolol, or atropine. Monosialoganglioside did not reduce ST-induced secretion. However, indomethacin and butylated hydroxyanisole decreased the ST effect on both guanylate cyclase and secretion. Fluid secretion with ST sppears to result from specific activation of particulate intestinal guanylate cyclase. While adrenergic and cholinergic events are probably not involved in this process, the effects of ST may be mediated through prostaglandin synthesis or oxidative mechnanisms.

Citations

Sep 1, 1995·Protein Science : a Publication of the Protein Society·M SukumarL M Gierasch
Dec 1, 1990·International Journal of Colorectal Disease·C P Conlon, T E Peto
Oct 15, 1986·Biochemical Pharmacology·S A WaldmanF Murad
Sep 4, 1992·European Journal of Pharmacology·E BeublerA Schirgi-Degen
Jan 1, 1984·Transactions of the Royal Society of Tropical Medicine and Hygiene·O M KorzeniowskiR L Guerrant
Jan 1, 1990·Toxicon : Official Journal of the International Society on Toxinology·F C KnoopW J Boehm
Jan 1, 1985·Pharmacology & Therapeutics·D C RobertsonF Dorner
Jan 1, 1991·Pharmacology & Therapeutics·D L Garbers
May 30, 1985·Biochimica Et Biophysica Acta·S A WaldmanF Murad
Apr 2, 1987·Biochimica Et Biophysica Acta·M M elDeibA A White
Sep 1, 1992·Journal of Pharmacological and Toxicological Methods·F C Knoop, M Owens
Dec 30, 1988·Biochemical and Biophysical Research Communications·M NakaneF Murad
Dec 1, 1985·The Journal of Hygiene·R J Gross, B Rowe
Aug 1, 1991·The New England Journal of Medicine·R L Guerrant, D A Bobak
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R J WinquistR M Rapoport
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M G CurrieC E Smith
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S J SavarinoP Guerry
Dec 1, 1995·The Journal of Experimental Medicine·G R KlimpelJ W Peterson
Mar 1, 1992·Pharmacology & Toxicology·A A LimaM C Fonteles
Jul 1, 1986·The Journal of Clinical Investigation·F Murad
Jun 27, 2012·Biomarkers in Medicine·Jian P GongScott A Waldman
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·G M PitariS A Waldman
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·S L CarrithersS A Waldman
Aug 6, 2011·Canadian Journal of Physiology and Pharmacology·Manassés Claudino Fonteles, Nilberto Robson Falcão do Nascimento
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J Gariépy, G K Schoolnik
Feb 14, 2008·Expert Opinion on Therapeutic Targets·Darren D Browning
Nov 10, 2009·Expert Review of Molecular Diagnostics·Alex MejiaScott A Waldman
Aug 27, 2013·Expert Review of Clinical Pharmacology·Erik S BlomainScott A Waldman
Jul 22, 2015·Expert Opinion on Drug Discovery·Pamela J Hornby
Nov 1, 1987·Archives internationales de physiologie et de biochimie·M Gilles-BaillienP Y Duchesne
Sep 1, 1987·The American Journal of the Medical Sciences·F MuradS A Waldman
Aug 1, 1985·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·E A Gonzalez, J Blanco
Mar 24, 2012·Journal of Surgical Oncology·Alex MejiaScott A Waldman
Jun 29, 2004·The American Journal of Cardiology·Ferid Murad

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