Activation of JNK and c-Jun is involved in glucose oxidase-mediated cell death of human lymphoma cells

Molecules and Cells
Young-Ok SonJeong-Chae Lee

Abstract

Mitogen-activated protein kinases (MAPK) affect the activation of activator protein-1 (AP-1), which plays an important role in regulating a range of cellular processes. However, the roles of these signaling factors on hydrogen peroxide (H(2)O(2))-induced cell death are unclear. This study examined the effects of H(2)O(2) on the activation of MAPK and AP-1 by exposing the cells to H(2)O(2) generated by either glucose oxidase or a bolus addition. Exposing BJAB or Jurkat cells to H(2)O(2) affected the activities of MAPK differently according to the method of H(2)O(2) exposure. H(2)O(2) increased the AP-1-DNA binding activity in these cells, where continuously generated H(2)O(2) led to an increase in mainly the c-Fos, FosB and c-Jun proteins. The c-Jun-NH(2)-terminal kinase (JNK)-mediated activation of c-Jun was shown to be related to the H(2)O(2)-induced cell death. However, the suppression of H(2)O(2)-induced oxidative stress by either JNK inhibitor or c-Jun specific antisense transfection was temporary in the cells exposed to glucose oxidase but not to a bolus H(2)O(2). This was associated with the disruption of death signaling according to the severe and prolonged depletion of reduced glutathione. Overall, these results suggest...Continue Reading

References

Oct 25, 1991·Nucleic Acids Research·T SuzukiH Iba
Nov 2, 1987·Biochimica Et Biophysica Acta·M Goppelt-Strübe, K Resch
Jan 1, 1983·Annual Review of Biochemistry·A Meister, M E Anderson
Nov 24, 1995·Science·Z XiaM E Greenberg
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·K YoshiokaM Karin
May 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C K Sen, L Packer
Jun 25, 1998·Molecular and Cellular Biology·S M Jacobs-HelberS T Sawyer
Mar 10, 2001·Nature·L Chang, M Karin
Apr 13, 2004·Oncogene·Sean P CreganRuth S Slack
Nov 27, 2004·Journal of Cell Science·Jochen HessMarina Schorpp-Kistner
Mar 15, 2006·Apoptosis : an International Journal on Programmed Cell Death·P SanchoP Aller
Jul 26, 2006·Free Radical Biology & Medicine·Derick HanNeil Kaplowitz
Oct 16, 2007·Free Radical Biology & Medicine·Alexandra BarboutiDimitrios Galaris
Jan 16, 2009·The FEBS Journal·Ankur MaheshwariDeoki Nandan
May 7, 2009·Apoptosis : an International Journal on Programmed Cell Death·Young-Ok SonJeong-Chae Lee

❮ Previous
Next ❯

Citations

Nov 5, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Young-Ok SonXianglin Shi
May 24, 2012·Oxidative Medicine and Cellular Longevity·Marlene Jimenez-Del-Rio, Carlos Velez-Pardo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.